DSpace Repository

Quantification of Leishmania (Viannia) Kinetoplast DNA in Ulcers of Cutaneous Leishmaniasis Reveals Inter-site and Inter-sampling Variability in Parasite Load

Show simple item record

dc.contributor.author Suarez, Milagros
dc.contributor.author Valencia, B. M.
dc.contributor.author Jara, Marlene
dc.contributor.author Alba, Milena
dc.contributor.author Boggild, Andrea K.
dc.contributor.author Dujardin, Jean-Claude
dc.contributor.author Llanos-Cuentas, Alejandro
dc.contributor.author Arevalo, Jorge
dc.contributor.author Adaui, Vanessa
dc.date.accessioned 2019-02-06T14:53:09Z
dc.date.available 2019-02-06T14:53:09Z
dc.date.issued 2015
dc.identifier.uri http://doi.org/10.1371/journal.pntd.0003936
dc.identifier.uri http://repositorio.upch.edu.pe/handle/upch/5385
dc.description.abstract BACKGROUND: Cutaneous leishmaniasis (CL) is a skin disease caused by the protozoan parasite Leishmania. Few studies have assessed the influence of the sample collection site within the ulcer and the sampling method on the sensitivity of parasitological and molecular diagnostic techniques for CL. Sensitivity of the technique can be dependent upon the load and distribution of Leishmania amastigotes in the lesion. METHODOLOGY/PRINCIPAL FINDINGS: We applied a quantitative real-time PCR (qPCR) assay for Leishmania (Viannia) minicircle kinetoplast DNA (kDNA) detection and parasite load quantification in biopsy and scraping samples obtained from 3 sites within each ulcer (border, base, and center) as well as in cytology brush specimens taken from the ulcer base and center. A total of 248 lesion samples from 31 patients with laboratory confirmed CL of recent onset (</=3 months) were evaluated. The kDNA-qPCR detected Leishmania DNA in 97.6% (242/248) of the examined samples. Median parasite loads were significantly higher in the ulcer base and center than in the border in biopsies (P<0.0001) and scrapings (P = 0.0002). There was no significant difference in parasite load between the ulcer base and center (P = 0.80, 0.43, and 0.07 for biopsy, scraping, and cytology brush specimens, respectively). The parasite load varied significantly by sampling method: in the ulcer base and center, the descending order for the parasite load levels in samples was: cytology brushes, scrapings, and biopsies (P<0.0001); in the ulcer border, scrapings had higher parasite load than biopsies (P<0.0001). There was no difference in parasite load according to L. braziliensis and L. peruviana infections (P = 0.4). CONCLUSION/SIGNIFICANCE: Our results suggest an uneven distribution of Leishmania amastigotes in acute CL ulcers, with higher parasite loads in the ulcer base and center, which has implications for bedside collection of diagnostic specimens. The use of scrapings and cytology brushes is recommended instead of the more invasive biopsy.
dc.language.iso eng
dc.publisher Public Library of Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Adolescent
dc.subject Adult
dc.subject Female
dc.subject Humans
dc.subject Male
dc.subject Young Adult
dc.subject Aged
dc.subject Middle Aged
dc.subject Species Specificity
dc.subject DNA, Kinetoplast/genetics
dc.subject Parasite Load
dc.subject Leishmania/classification/genetics
dc.subject Leishmaniasis, Cutaneous/parasitology/pathology
dc.subject Skin Ulcer/parasitology/pathology
dc.title Quantification of Leishmania (Viannia) Kinetoplast DNA in Ulcers of Cutaneous Leishmaniasis Reveals Inter-site and Inter-sampling Variability in Parasite Load
dc.type info:eu-repo/semantics/article
dc.identifier.journal PLoS Neglected Tropical Diseases


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics