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C57BL/6 α-1,3-Galactosyltransferase knockout mouse as an animal model for experimental Chagas disease

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dc.contributor.author Valencia Ayala, Edward
dc.contributor.author Rodrigues da Cunha, Gisele
dc.contributor.author Araujo Azevedo, Maira
dc.contributor.author Calderon, Maritza
dc.contributor.author Jimenez, Juan
dc.contributor.author Venuto, Ana Paula
dc.contributor.author Gazzinelli, Ricardo
dc.contributor.author Ynocente Lavalle, Raúl Jesus
dc.contributor.author Vidal Riva, Angela Giovana
dc.contributor.author Hincapie, Robert
dc.contributor.author Finn, M. G.
dc.contributor.author Marques, Alexandre F.
dc.date.accessioned 2020-07-14T00:02:36Z
dc.date.available 2020-07-14T00:02:36Z
dc.date.issued 2020
dc.identifier.uri http://repositorio.upch.edu.pe/handle/upch/8355
dc.description.abstract The leading animal model of experimental Chagas disease, the mouse, plays a significant role in studies for vaccine development, diagnosis, and human therapies. Humans, along with Old World primates, alone among mammals, cannot make the terminal carbohydrate linkage of the α-Gal trisaccharide. It has been established that the anti-α-Gal immune response is likely to be a critical factor for protection against Trypanosoma cruzi (T. cruzi) infection in humans. However, the mice customarily employed for the study of T. cruzi infection naturally express the α-Gal epitope and therefore do not produce anti-α-Gal antibodies. Here, we used the C57BL/6 α-1,3-galactosyltransferase knockout (α-GalT-KO) mouse, which does not express the α-Gal epitope as a model for experimental Chagas disease. We found the anti-α-Gal IgG antibody response to an increase in α-GalT-KO mice infected with Arequipa and Colombiana strains of T. cruzi, leading to fewer parasite nests, lower parasitemia, and an increase of INF-γ, TNF-α, and IL-12 cytokines in the heart of α-GalT-KO mice compared with α-GalT-WT mice on days 60 and 120 postinfection. We therefore agree that the C57BL/6 α-GalT-KO mouse represents a useful model for initial testing of therapeutic and immunological approaches against different strains of T. cruzi. en_US
dc.language.iso eng
dc.publisher American Chemical Society
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Chagas disease en_US
dc.subject mouse model en_US
dc.subject Trypanosoma cruzi en_US
dc.subject α-Gal en_US
dc.subject α-Gal antibodies en_US
dc.subject α-Gal knockout mouse en_US
dc.title C57BL/6 α-1,3-Galactosyltransferase knockout mouse as an animal model for experimental Chagas disease en_US
dc.type info:eu-repo/semantics/article
dc.identifier.journal ACS Infectious Diseases
dc.identifier.doi https://doi.org/10.1021/acsinfecdis.0c00061

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