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Browsing by Subject "Molecular Diagnostic Techniques"

Browsing by Subject "Molecular Diagnostic Techniques"

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  • Development of a novel protocol based on blood clot to improve the sensitivity of qPCR detection of toxoplasma gondii in peripheral blood specimens 
    Gutierrez Loli, Renzo Marcelo; Ferradas, C.; Diestra, A.; Traianou, A.; Bowman, N.; Bok, J.; Reimer-McAtee, M.; Ramal, C.; Ticona, E.; Steinberg, H.; Mayta, H.; Calderón Sánchez, Maritza Mercedes; Calla Choque, Jaeson Santos; Sterling, C.; Gilman, Robert Hugh; Pinedo, L.C.; Valencia, G.; Sanchez, L.; Málaga, E.; Zhu, D.; Jiménez, J.; Bern, C.; Angulo, N.; Schiaffino Salazar, Francesca; Acosta, J.; Holtz, M.; Clark, D.; Clark, T.; Trompeter, G.; Choi, J.; Gandarilla, O.; Dorn, M.; Fortuny, E.; Galdos, G.; Colanzi, R. (American Society of Tropical Medicine and Hygiene, 2019)
    Quantitative polymerase chain reaction (qPCR) for Toxoplasma gondii multicopy genes has emerged as a promising strategy for sensitive detection of parasite DNA. qPCR can be performed from blood samples, which are minimally ...
  • Estandarización y validación de una prueba molecular RT-LAMP in house para el diagnóstico de SARS-CoV-2 
    Escalante-Maldonado, O.; Vidal-Anzardo, M.; Donaires, F.; Solis-Sanchez, G.; Gallesi, I.; Pampa-Espinoza, L.; Huaringa, M.; Rojas-Serrano, N.; García Apac, Coralith Marlinda; Angles-Yanqui, E.; Gavilán, R.G.; Durães-Carvalho, R.; Mendez-Rico, J.; Cabezas, C.; Marques-Simas, P.V. (Instituto Nacional de Salud, 2021)
    OBJECTIVES: To standardize and validate an in-house RT-LAMP test for the detection of SARS-CoV-2, based on laboratory and field assays using samples from COVID-19 suspected patients. MATERIALS AND METHODS: An in-house ...
  • Improved DNA extraction technique from clot for the diagnosis of Chagas disease 
    Mayta, Holger; Romero, Yomara K.; Pando, Alejandra; Verastegui Pimentel, Manuela Renee; Tinajeros, Freddy; Bozo, Ricardo; Henderson-Frost, Josephine; Colanzi, Rony; Flores, Jorge; Lerner, Richard; Bern, Caryn; Gilman, Robert Hugh (Public Library of Science, 2019)
    BACKGROUND: The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater ...
  • Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach 
    Veland, N.; Boggild, A.K.; Valencia, C.; Valencia, B.M.; Llanos Cuentas, Elmer Alejandro; Van Der Auwera, G.; Dujardin, J.-C.; Arévalo Zelada, Jorge Luis (American Society for Microbiology, 2012)
    We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered ...
  • Multiplex real-time PCR for detection of Campylobacter, Salmonella, and Shigella 
    Barletta, F.; Mercado, E. H.; Lluque, A.; Ruiz, J.; Cleary, T. G.; Ochoa Woodell, Theresa Jean (American Society for Microbiology, 2013)
    Infectious diarrhea can be classified based on its clinical presentation as noninflammatory or inflammatory disease. In developing countries, among inflammatory diarrhea cases, Shigella is the most common cause, followed ...
  • Non-invasive cytology brush pcr diagnostic testing in mucosal leishmaniasis: Superior performance to conventional biopsy with histopathology 
    Boggild, A.K.; Valencia, B.M.; Veland, N.; Ramos, A.P.; Calderon, F.; Arévalo Zelada, Jorge Luis; Low, D.E.; Llanos Cuentas, Elmer Alejandro (Public Library of Science, 2011)
    Background: Traditional methods of diagnosing mucosal leishmaniasis (ML), such as biopsy with histopathology, are insensitive and require collection of an invasive diagnostic specimen. Methods: We compared standard invasive ...
  • Novel bartonella agent as cause of verruga peruana 
    Blazes, David L.; Mullins, Kristin; Smoak, BonnieL.; Jiang, Ju; Canal, Enrique; Solorzano, Nelson; Hall, Eric; Meza, Rina; Maguiña Vargas, Ciro; Myers, Todd; Richards, Allen L.; Laughlin, Larry (Centers for Disease Control and Prevention, 2013)
    While studying chronic verruga peruana infections in Peru from 2003, we isolated a novel Bartonella agent, which we propose be named Candidatus Bartonella ancashi. This case reveals the inherent weakness of relying solely ...
  • A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis 
    Juscamayta López, Julio Eduardo; Valdivia, F.; Soto, M.P.; Nureña, B.; Horna, H. (Nature Research, 2023)
    Despite widespread vaccination, Bordetella pertussis continues to cause pertussis infections worldwide, leaving infants at the highest risk of severe illness and death, while people around them are likely the main sources ...

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