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Browsing by Subject "molecular diagnosis"

Browsing by Subject "molecular diagnosis"

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  • Cost avoidance and outcome improvement by implementing a test utilization strategy for molecular microbiology tests 
    Ricaldi, Jessica; Racsa, Lori; Pusch, Tobias; Gander, Rita M.; Southern, Paul; Cavuoti, Dominick; Byrd, Linda; Alatoom, Adnan (Elsevier, 2014)
    Test utilization is an essential strategy in the clinical laboratory, especially with increasing numbers of tests and health care costs. We designed a triage system for molecular microbiology tests ordered from 2007 through ...
  • Development of a novel protocol based on blood clot to improve the sensitivity of qPCR detection of toxoplasma gondii in peripheral blood specimens 
    Gutierrez Loli, Renzo Marcelo; Ferradas, C.; Diestra, A.; Traianou, A.; Bowman, N.; Bok, J.; Reimer-McAtee, M.; Ramal, C.; Ticona, E.; Steinberg, H.; Mayta, H.; Calderón Sánchez, Maritza Mercedes; Calla Choque, Jaeson Santos; Sterling, C.; Gilman, Robert Hugh; Pinedo, L.C.; Valencia, G.; Sanchez, L.; Málaga, E.; Zhu, D.; Jiménez, J.; Bern, C.; Angulo, N.; Schiaffino Salazar, Francesca; Acosta, J.; Holtz, M.; Clark, D.; Clark, T.; Trompeter, G.; Choi, J.; Gandarilla, O.; Dorn, M.; Fortuny, E.; Galdos, G.; Colanzi, R. (American Society of Tropical Medicine and Hygiene, 2019)
    Quantitative polymerase chain reaction (qPCR) for Toxoplasma gondii multicopy genes has emerged as a promising strategy for sensitive detection of parasite DNA. qPCR can be performed from blood samples, which are minimally ...
  • A diagnostic accuracy study of Xpert®MTB/RIF in HIV-positive patients with high clinical suspicion of pulmonary tuberculosis in Lima, Peru. 
    Carriquiry, Gabriela; Otero Vegas, Larissa; González Lagos, Elsa Violeta; Zamudio Fuertes, Carlos Eduardo; Sánchez, Eduardo; Nabeta, Pamela; Campos, Miguel; Echevarria Zarate, Juan Ignacio; Seas Ramos, Carlos Rafael; Gotuzzo Herencia, José Eduardo (Public Library of Science, 2012)
    Background: Diagnosis of pulmonary tuberculosis (TB) among human immunodeficiency virus (HIV) patients remains complex and demands easy to perform and accurate tests. Xpert®MTB/RIF (MTB/RIF) is a molecular TB diagnostic ...
  • Estandarización y validación de una prueba molecular RT-LAMP in house para el diagnóstico de SARS-CoV-2 
    Escalante-Maldonado, O.; Vidal-Anzardo, M.; Donaires, F.; Solis-Sanchez, G.; Gallesi, I.; Pampa-Espinoza, L.; Huaringa, M.; Rojas-Serrano, N.; García Apac, Coralith Marlinda; Angles-Yanqui, E.; Gavilán, R.G.; Durães-Carvalho, R.; Mendez-Rico, J.; Cabezas, C.; Marques-Simas, P.V. (Instituto Nacional de Salud, 2021)
    OBJECTIVES: To standardize and validate an in-house RT-LAMP test for the detection of SARS-CoV-2, based on laboratory and field assays using samples from COVID-19 suspected patients. MATERIALS AND METHODS: An in-house ...
  • Improved DNA extraction technique from clot for the diagnosis of Chagas disease 
    Mayta, Holger; Romero, Yomara K.; Pando, Alejandra; Verastegui Pimentel, Manuela Renee; Tinajeros, Freddy; Bozo, Ricardo; Henderson-Frost, Josephine; Colanzi, Rony; Flores, Jorge; Lerner, Richard; Bern, Caryn; Gilman, Robert Hugh (Public Library of Science, 2019)
    BACKGROUND: The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater ...
  • MLPA followed by target-NGS to detect mutations in the dystrophin gene of Peruvian patients suspected of DMD/DMB 
    Guevara-Fujita, Maria Luisa; Huaman-Dianderas, Francia; Obispo, Daisy; Sanchez, Rodrigo; Barrenechea, Victor; Rojas-Malaga, Diana; Estrada-Cuzcano, Alejandro; Trubnykova, Milana; Cornejo-Olivas, Mario; Marca, Victoria; Gallardo, Bertha; Duenas-Roque, Milagros; Protzel, Ana; Castaneda, Carlos; Abarca, Hugo; Celis, Luis; La Serna-Infantes, Jorge; Fujita, Ricardo (Wiley, 2021)
    Background: We report the molecular analysis of the DMD gene in a group of Peruvian patients with Duchenne/Becker dystrophinopathy. This is the first study to thoroughly characterize mutations in this population.Methods: ...
  • Mycobacterium abscessus Complex Cutaneous Infection 
    Porudominsky, R.; Gotuzzo Herencia, José Eduardo (Springer, 2018)
    Purpose of Review: The worldwide increase in skin infections due to Mycobacterium abscessus, especially in Latin America, and its difficult diagnosis and treatment, makes a review of literature crucial in reminding and ...
  • Neurogenetics in Peru: clinical, scientific and ethical perspectives 
    Cornejo-Olivas, M.; Espinoza Huertas, Keren Antonieta; Velit-Salazar, M.R.; Veliz-Otani, D.; Tirado-Hurtado, I.; Inca-Martinez, M.; Silva-Paredes, G.; Milla-Neyra, K.; Marca, V.; Ortega, O.; Mazzetti, P. (Springer, 2015)
    Neurogenetics, the science that studies the genetic basis of the development and function of the nervous system, is a discipline of recent development in Peru, an emerging Latin American country. Herein, we review the ...
  • Non-invasive cytology brush pcr diagnostic testing in mucosal leishmaniasis: Superior performance to conventional biopsy with histopathology 
    Boggild, A.K.; Valencia, B.M.; Veland, N.; Ramos, A.P.; Calderon, F.; Arévalo Zelada, Jorge Luis; Low, D.E.; Llanos Cuentas, Elmer Alejandro (Public Library of Science, 2011)
    Background: Traditional methods of diagnosing mucosal leishmaniasis (ML), such as biopsy with histopathology, are insensitive and require collection of an invasive diagnostic specimen. Methods: We compared standard invasive ...
  • Quinolone Resistance in Absence of Selective Pressure: The Experience of a Very Remote Community in the Amazon Forest 
    Pallecchi, L.; Bartoloni, A.; Riccobono, E.; Fernandez, C.; Mantella, A.; Magnelli, D.; Mannini, D.; Strohmeyer, M.; Bartalesi, F.; Rodriguez, H.; Gotuzzo Herencia, José Eduardo; Rossolini, G.M. (Public Library of Science, 2012)
    Background: Quinolones are potent broad-spectrum bactericidal agents increasingly employed also in resource-limited countries. Resistance to quinolones is an increasing problem, known to be strongly associated with quinolone ...

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