Listar por autor "Ruiz, Joaquim"

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Azithromycin resistance levels and mechanisms in Escherichia coli

Gomes, Claudia; Ruiz-Roldan, Lidia; Mateu, Judit; Ochoa Woodell, Theresa Jean; Ruiz, Joaquim (Springer Nature, 2019)

Despite azithromycin being used in some countries to treat infections caused by Gram-negative pathogens, no resistance breakpoint for Escherichia coli exists. The aim of this study was to analyse the levels and mechanisms ...
Caracterización de Escherichia coli D7111 productora de β-lactamasa TEM-176

Ruiz, Joaquim; Pons, Maria J.; Mosquito, Susan; Ochoa Woodell, Theresa Jean; Sáenz, Yolanda (Instituto Nacional de Salud, 2021)

The present report is the original description of blaTEM-176. The mechanisms of resistance to antimicrobial agents were determined in an enterotoxigenic Escherichia coli, determining the susceptibility to 22 antimicrobials ...
Characterisation of the first KPC-2-producing Klebsiella pneumoniae ST340 from Peru

Horna, Gertrudis; Velasquez, Jorge; Fernandez, Nathaly; Tamariz, Jesus; Ruiz, Joaquim (Elsevier, 2017)

OBJECTIVES: The aim of this study was to characterise a KPC-carrying Klebsiella pneumoniae isolate from a Peruvian hospital setting. METHODS: The identity of the isolate was confirmed by amplification and sequencing of the ...
Class 1 and 2 Integrons in Escherichia coli Strains Isolated from Diarrhea and Bacteremia in Children Less Than 2 Years of Age from Peru.

Riveros Ramirez, Maribel Denise; Pons, Maria J.; Durand Vara, David Percy; Ochoa Woodell, Theresa Jean; Ruiz, Joaquim (American Society of Tropical Medicine and Hygiene, 2023)

Class 1 and Class 2 integrons are mobilizable elements able to carry a variety of antibiotic resistance determinants. In the present study, Class 1 and 2 integrons present in 355 pathogenic Escherichia coli (285 diarrheagenic, ...
Colonizing and Virulence Factors in Enterotoxigenic Escherichia coli from Peru.

Rivera Albinagorta, Fulton Paul; Medina Prada, Anicia Magaly; Riveros Ramirez, Maribel Denise; Ochoa Woodell, Theresa Jean; Pons, Maria J.; Ruiz, Joaquim (American Journal of Tropical Medicine and Hygiene, 2023)

Enterotoxigenic Escherichia coli (ETEC) ranks among the most relevant diarrheagenic pathogens. Efforts to design vaccines to fight ETEC have been focused on colonizing factors (CFs) and atypical virulence factors (AVF). ...
Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru

Medina, Anicia M.; Rivera, Fulton P.; Pons, Maria J.; Riveros Ramirez, Maribel Denise; Gomes, Claudia; Bernal, Maria; Meza, Rina; Maves, Ryan C.; Huicho Oriundo, Luis; Chea-Woo, Elsa; Lanata, Claudio F.; Gil, Ana I.; Ochoa Woodell, Theresa Jean; Ruiz, Joaquim (Oxford University Press, 2015)

BACKGROUND: Antibiotic resistance is increasing worldwide, being of special concern in low- and middle-income countries. The aim of this study was to determine the antimicrobial susceptibility and mechanisms of resistance ...
Comparison of bacterial load of enteroaggregative Escherichia coli (EAEC) by real-time PCR (qPCR) in children under 2 years of age with or without diarrhea from a peri-urban area of Lima, Peru.

Durand, David; Mercado, Erik; Ruiz, Joaquim; Ochoa, Teresa (Universidad Peruana Cayetano Heredia, 2019)

Introduction: Enteroagregative Escherichia coli (EAEC) is a group of enteric pathogens that cause acute and persistent diarrhea among children, in patients infected with human immunodeficiency virus, which causes traveler's ...
Detection of secretory immunoglobulin a in human colostrum as mucosal immune response against proteins of the type III secretion system of Salmonella, Shigella and enteropathogenic Escherichia Coli

Durand Vara, David Percy; Ochoa Woodell, Theresa Jean; Bellomo, Sicilia M. E.; Contreras, Carmen A.; Bustamante, Víctor H.; Ruiz, Joaquim; Cleary, Thomas G. (Wolters Kluwer Health, 2013)

Some enteropathogens use the type III secretion system to secrete proteins that allows them to interact with enterocytes and promote bacterial attachment or intracellular survival. These proteins are Salmonella invasion ...
Development and analysis of furazolidone-resistant Escherichia coli mutants

Martinez-Puchol, Sandra; Gomes, Claudia; Pons, Maria J.; Ruiz-Roldan, Lidia; Torrents de la Pena, Alba; Ochoa Woodell, Theresa Jean; Ruiz, Joaquim (Wiley, 2015)

Furazolidone-resistant mutants were obtained from four clinical isolates of diarrhoeagenic Escherichia coli. The stability of the resistance and the frequency of mutation were established. The minimal inhibitory concentration ...
Diarrheagenic Escherichia coli phylogroups are associated with antibiotic resistance and duration of diarrheal episode

Mosquito, Susan; Pons, Maria J.; Riveros Ramirez, Maribel Denise; Ruiz, Joaquim; Ochoa Woodell, Theresa Jean (Hindawi, 2015)

Conventionally, in Escherichia coli, phylogenetic groups A and B1 are associated with commensal strains while B2 and D are associated with extraintestinal strains. The aim of this study was to evaluate diarrheagenic (DEC) ...
Dissemination of a multidrug resistant CTX-M-65 producer Salmonella enterica serovar Infantis clone between marketed chicken meat and children

Martínez-Puchol, Sandra; Riveros Ramirez, Maribel Denise; Ruidias, Kenny; Granda, Ana; Ruiz-Roldán, Lidia; Zapata-Cachay, Cristhian; Ochoa Woodell, Theresa Jean; Pons, Maria J.; Ruiz, Joaquim (Elsevier, 2021)

The objective of the present study was to characterize Salmonella enterica serovar Infantis isolated from chicken meat determining their clonal relationships with S. Infantis isolated from children with diarrhea. Fifteen ...
Factors affecting caregivers' use of antibiotics available without a prescription in Peru

Ecker, Lucie; Ochoa Woodell, Theresa Jean; Vargas, Martha; Del Valle, Luis J.; Ruiz, Joaquim (American Academy of Pediatrics, 2013)

OBJECTIVE: To determine factors that affect caregivers’ decisions about antibiotic use in children in settings where antibiotics are available without prescription. METHODS: In a house-to-house survey, 1200 caregivers in ...
Feeding of young children during diarrhea: caregivers' intended practices and perceptions

Pantenburg, Birte; Ochoa Woodell, Theresa Jean; Ecker, Lucie; Ruiz, Joaquim (American Society of Tropical Medicine and Hygiene, 2014)

Childhood diarrhea is an important cause of malnutrition, which can be worsened when caretakers limit nutritional support. We queried 390 caregivers and their children in a peri-urban community in Lima, Peru regarding ...
Frecuencia de genes que codifican proteínas autotransportadoras serinproteasa de Enterobacteriaceae (SPATE) en cepas de Escherichia coli difusamente adherente (DAEC) provenientes de niños con y sin diarrea

Ocampo, Karen; Riveros Ramirez, Maribel Denise; Pinedo-Bardales, Maria; Ruiz, Joaquim; Ochoa Woodell, Theresa Jean (Instituto Nacional de Salud, 2021)

The aim of the study was to determine the frequency of six genes encoding serine protease autotransporter proteins Enterobacteriaceae (SPATE) in diffusely adherent Escherichia coli (DAEC) isolates from children with (WD, ...
Frecuencia y patotipos de Escherichia coli diarrogénicas en niños peruanos con y sin diarrea

Ochoa Woodell, Theresa Jean; Mercado, E.H.; Durand Vara, David Percy; Rivera, F.P.; Mosquito, S.; Contreras, C.; Riveros Ramirez, Maribel Denise; Lluque, A.; Barletta, F.; Prada, A.; Ruiz, Joaquim (Instituto Nacional de Salud, 2011)

Introduction. Diarrheagenic E. coli (DEC) are a major cause of diarrhea in children in developing countries. However, they are not part of routine diagnosis in clinical laboratories. Objectives. To determine the DEC ...
Genotypic characterization of enterotoxigenic escherichia coli strains causing traveler's diarrhea

Rivera, Fulton P.; Medina, Anicia M.; Aldasoro, Edelweiss; Sangil, Anna; Gascon, Joaquim; Ochoa Woodell, Theresa Jean; Vila, Jordi; Ruiz, Joaquim (American Society for Microbiology, 2013)

This study aims to characterize the presence of virulence factors of enterotoxigenic Escherichia coli (ETEC) causing traveler's diarrhea. Among 52 ETEC isolates, the most common toxin type was STh, and the most frequent ...
High frequency of the exoU+/exoS+ genotype associated with multidrug-resistant "high-risk clones" of Pseudomonas aeruginosa clinical isolates from Peruvian hospitals

Horna, Gertrudis; Amaro, Catherine; Palacios, Aida; Guerra, Humberto; Ruiz, Joaquim (Springer Nature, 2019)

The type III secretion system of Pseudomonas aeruginosa is an important virulence factor contributing to the cytotoxicity and the invasion process of this microorganism. The current study aimed to determine the presence ...
High Prevalence of blaCTX-Min Fecal Commensal Escherichia coli from Healthy Children

Alcedo, Katherine; Ruiz, Joaquim; Ochoa Woodell, Theresa Jean; Riveros Ramirez, Maribel Denise (Korean Society of Infectious Diseases, 2022)

Background: Antibiotic-resistant Escherichia coli can colonize the intestinal tract of healthy children, causing concern when antibiotic resistance is related to the presence of transferable mechanisms, such as extended-spectrum ...
In vitro development and analysis of escherichia coli and shigella boydii azithromycin-resistant mutants

Gomes, Claudia; Pons, Maria J.; Magallon-Tejada, Ariel; Durand Vara, David Percy; Lluque, Angela; Mosquito, Susan; Riveros Ramirez, Maribel Denise; Mercado, Erik; Prada, Ana; Ochoa Woodell, Theresa Jean; Ruiz, Joaquim (Mary Ann Liebert, 2013)

Three clinical isolates of E. coli and one S. boydii isolated from feces samples collected from children under 5 years of age with diarrhea in Lima, Peru were inoculated onto Mueller-Hinton plates containing increasing ...
Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa

Horna, Gertrudis; López, María; Guerra, Humberto; Saénz, Yolanda; Ruiz, Joaquim (Springer Nature, 2018)

MexAB-OprM and MexEF-OprN are Pseudomonas aeruginosa efflux pumps involved in the development of antibiotic resistance. Several studies developed with laboratory strains or using a few clinical isolates have reported that ...