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  • Development of a novel protocol based on blood clot to improve the sensitivity of qPCR detection of toxoplasma gondii in peripheral blood specimens 
    Gutierrez Loli, Renzo Marcelo; Ferradas, C.; Diestra, A.; Traianou, A.; Bowman, N.; Bok, J.; Reimer-McAtee, M.; Ramal, C.; Ticona, E.; Steinberg, H.; Mayta, H.; Calderón Sánchez, Maritza Mercedes; Calla Choque, Jaeson Santos; Sterling, C.; Gilman, Robert Hugh; Pinedo, L.C.; Valencia, G.; Sanchez, L.; Málaga, E.; Zhu, D.; Jiménez, J.; Bern, C.; Angulo, N.; Schiaffino Salazar, Francesca; Acosta, J.; Holtz, M.; Clark, D.; Clark, T.; Trompeter, G.; Choi, J.; Gandarilla, O.; Dorn, M.; Fortuny, E.; Galdos, G.; Colanzi, R. (American Society of Tropical Medicine and Hygiene, 2019)
    Quantitative polymerase chain reaction (qPCR) for Toxoplasma gondii multicopy genes has emerged as a promising strategy for sensitive detection of parasite DNA. qPCR can be performed from blood samples, which are minimally ...
  • Improved DNA extraction technique from clot for the diagnosis of Chagas disease 
    Mayta, Holger; Romero, Yomara K.; Pando, Alejandra; Verastegui Pimentel, Manuela Renee; Tinajeros, Freddy; Bozo, Ricardo; Henderson-Frost, Josephine; Colanzi, Rony; Flores, Jorge; Lerner, Richard; Bern, Caryn; Gilman, Robert Hugh (Public Library of Science, 2019)
    BACKGROUND: The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater ...

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