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Detection of dengue virus serotype 3 in Cajamarca, Peru: Molecular diagnosis and clinical characteristics

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dc.contributor.author Aguilar-Luis, M.
dc.contributor.author Carrillo-Ng, H.
dc.contributor.author Kym, S.
dc.contributor.author Silva-Caso, W.
dc.contributor.author Verne, E.
dc.contributor.author Del Valle, L.
dc.contributor.author Bazn-Mayra, J.
dc.contributor.author Zavaleta-Gavidia, V.
dc.contributor.author Cornejo-Pacherres, D.
dc.contributor.author Tarazona-Castro, Y.
dc.contributor.author Aquino-Ortega, R.
dc.contributor.author Cornejo-Tapia, A.
dc.contributor.author Valle-Mendoza, J.
dc.date.accessioned 2021-12-12T20:24:57Z
dc.date.available 2021-12-12T20:24:57Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/10260
dc.description.abstract Objective: To describe and molecularly characterize an outbreak of dengue virus (DENV) infection in Cajamarca, an Andean region in Peru. Methods: A total of 359 serum samples from patients with acute febrile illness were assessed for the presence of DENV via RT-PCR, ELISA NS1, IgM and IgG in Cajamarca, Peru from January 2017 to June 2017. The evaluation of the different diagnostic tests and their applicability was performed. Results: Dengue virus was detected in 24.7% of samples by RTPCR. Meanwhile, serological analysis detected 30.3% positive cases via ELISA NS1 antigen, 16.7% via ELISA IgG and 9.7% via ELISA IgM. Most of the cases corresponded to DENV-3 (77.5%). The use of RT-PCR performed better in primary infections (P<0.01), while detection of ELISA IgM performed better in secondary infections (P<0.01). The combination of NS1 and IgM performed better than the other assays in detecting primary (92.5%) and secondary infections (96.6%). The most frequent symptoms associated with fever were headaches, myalgias, and arthralgias across all groups. Conclusions: We report an important outbreak of dengue infection caused by DENV-3 in Cajamarca, Peru. Our findings encourage the use of NS1 antigen and IgM co-detection. These findings demonstrate an increasing expansion of DENV-3 in Peru and highlight the importance of molecular diagnosis and serotype characterization among the clinically defined dengue cases to strengthen the Peruvian epidemiological surveillance en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Asian Pacific Journal of Tropical Medicine
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Arbovirus en_US
dc.subject Dengue en_US
dc.subject DENV en_US
dc.subject Diagnosis en_US
dc.subject Peru en_US
dc.title Detection of dengue virus serotype 3 in Cajamarca, Peru: Molecular diagnosis and clinical characteristics en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4103/1995-7645.326257
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 2352-4146


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