Universidad Peruana Cayetano Heredia

Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm

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dc.contributor.author Millones Gómez, P.A.
dc.contributor.author Tay Chu Jon, Lidia Yileng
dc.contributor.author Maurtua Torres, Dora Jesus
dc.contributor.author Bacilio Amaranto, R.E.
dc.contributor.author Collantes Díaz, I.E.
dc.contributor.author Minchón Medina, C.A.
dc.date.accessioned 2021-12-12T20:24:57Z
dc.date.available 2021-12-12T20:24:57Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/10267
dc.description.abstract Background: Natural products with antibacterial potential have begun to be tested on biofilm models, bringing us closer to understanding the response generated by the complex microbial ecosystems of the oral cavity. The objective of this study was to evaluate the antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro biofilm of Streptococcus gordonii and Fusobacterium nucleatum. Methods: The experimental work involved a consecutive, in vitro, longitudinal, and double-blinded study design. Propolis samples were collected from 13 different regions of the Peruvian Andes. The disk diffusion method was used for the antimicrobial susceptibility test. The cytotoxic effect of propolis on human gingival fibroblasts was determined by cell viability method using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and the effect of propolis on the biofilm was evaluated by confocal microscopy and polymerase chain reaction (PCR). Results: The 0.78 mg/mL and 1.563 mg/mL concentrations of the methanolic fraction of the chloroform residue of Oxapampa propolis showed effects on biofilm thickness and the copy numbers of the srtA gene of S. gordonii and the radD gene of F. nucleatum at 48 and 120 hours, and chromatography (UV, λ 280 nm) identified rhamnocitrin, isorhamnetin, apigenin, kaempferol, diosmetin, acacetin, glycerol, and chrysoeriol. Conclusions: Of the 13 propolis evaluated, it was found that only the methanolic fraction of Oxapampa propolis showed antibacterial and antibiofilm effects without causing damage to human gingival fibroblasts. Likewise, when evaluating the chemical composition of this fraction, eight flavonoids were identified en_US
dc.language.iso eng
dc.publisher F1000 Research
dc.relation.ispartofseries F1000Research
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Antibacterial en_US
dc.subject Antibiofilm en_US
dc.subject Chemical compositions en_US
dc.subject Oral biofilm en_US
dc.subject Propolis en_US
dc.title Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.12688/f1000research.73602.1
dc.relation.issn 2046-1402


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