Store-operated channels in the pulmonary circulation of high- and low-altitude neonatal lambs

Parer, Julian T.; Reyes, Roberto V.; Moraga, Fernanda; Ferrada, Javiera; Ebensperger, Germán; Rojas, Rodrigo T.; Llanos, Aníbal J.; Hernandez, Ismael; Cabello, Gertrudis; Silva, Pablo; Herrera, Emilio A.; Parrau, Daniela; Diaz, Marcela; Riquelme, Raquel A.; Ulloa, César E.

dc.contributor.author	Parrau, Daniela
dc.contributor.author	Ebensperger, Germán
dc.contributor.author	Herrera, Emilio A.
dc.contributor.author	Moraga, Fernanda
dc.contributor.author	Riquelme, Raquel A.
dc.contributor.author	Ulloa, César E.
dc.contributor.author	Rojas, Rodrigo T.
dc.contributor.author	Silva, Pablo
dc.contributor.author	Hernandez, Ismael
dc.contributor.author	Ferrada, Javiera
dc.contributor.author	Diaz, Marcela
dc.contributor.author	Parer, Julian T.
dc.contributor.author	Cabello, Gertrudis
dc.contributor.author	Llanos, Aníbal J.
dc.contributor.author	Reyes, Roberto V.
dc.date.accessioned	2022-01-04T20:29:53Z
dc.date.available	2022-01-04T20:29:53Z
dc.date.issued	2013
dc.identifier.uri	https://hdl.handle.net/20.500.12866/10365
dc.description.abstract	We determined whether store-operated channels (SOC) are involved in neonatal pulmonary artery function under conditions of acute and chronic hypoxia, using newborn sheep gestated and born either at high altitude (HA, 3,600 m) or low altitude (LA, 520 m). Cardiopulmonary variables were recorded in vivo, with and without SOC blockade by 2-aminoethyldiphenylborinate (2-APB), during basal or acute hypoxic conditions. 2-APB did not have effects on basal mean pulmonary arterial pressure (mPAP), cardiac output, systemic arterial blood pressure, or systemic vascular resistance in both groups of neonates. During acute hypoxia 2-APB reduced mPAP and pulmonary vascular resistance in LA and HA, but this reduction was greater in HA. In addition, isolated pulmonary arteries mounted in a wire myograph were assessed for vascular reactivity. HA arteries showed a greater relaxation and sensitivity to SOC blockers than LA arteries. The pulmonary expression of two SOC-forming subunits, TRPC4 and STIM1, was upregulated in HA. Taken together, our results show that SOC contribute to hypoxic pulmonary vasoconstriction in newborn sheep and that SOC are upregulated by chronic hypoxia. Therefore, SOC may contribute to the development of neonatal pulmonary hypertension. We propose SOC channels could be potential targets to treat neonatal pulmonary hypertension.	en_US
dc.language.iso	eng
dc.publisher	American Physiological Society
dc.relation.ispartofseries	American Journal of Physiology - Lung Cellular and Molecular Physiology
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	animal	en_US
dc.subject	genetics	en_US
dc.subject	unclassified drug	en_US
dc.subject	controlled study	en_US
dc.subject	priority journal	en_US
dc.subject	altitude disease	en_US
dc.subject	Altitude Sickness	en_US
dc.subject	blood	en_US
dc.subject	physiology	en_US
dc.subject	altitude	en_US
dc.subject	arterial pressure	en_US
dc.subject	lung artery pressure	en_US
dc.subject	pulmonary artery	en_US
dc.subject	anoxia	en_US
dc.subject	pathophysiology	en_US
dc.subject	article	en_US
dc.subject	hypoxia	en_US
dc.subject	Infant, Newborn	en_US
dc.subject	newborn	en_US
dc.subject	drug effect	en_US
dc.subject	ex vivo study	en_US
dc.subject	disease model	en_US
dc.subject	in vivo study	en_US
dc.subject	Sheep, Domestic	en_US
dc.subject	2-aminoethyl diphenylborinate	en_US
dc.subject	blood vessel reactivity	en_US
dc.subject	boron derivative	en_US
dc.subject	calcium channel	en_US
dc.subject	cardiopulmonary function	en_US
dc.subject	heart output	en_US
dc.subject	hemodynamics	en_US
dc.subject	hypoxic lung vasoconstriction	en_US
dc.subject	ion channel	en_US
dc.subject	Ion Channels	en_US
dc.subject	isolated organ	en_US
dc.subject	lamb	en_US
dc.subject	lung circulation	en_US
dc.subject	lung vascular resistance	en_US
dc.subject	Persistent Fetal Circulation Syndrome	en_US
dc.subject	persistent pulmonary hypertension	en_US
dc.subject	Pulmonary Circulation	en_US
dc.subject	Pulmonary hypertension	en_US
dc.subject	Pulmonary vascular reactivity	en_US
dc.subject	Pulmonary vasoconstriction	en_US
dc.subject	sheep	en_US
dc.subject	store operated channel	en_US
dc.subject	systemic vascular resistance	en_US
dc.subject	transient receptor potential channel C	en_US
dc.subject	TRPC Cation Channels	en_US
dc.subject	TRPC4 ion channel	en_US
dc.subject	vasoconstriction	en_US
dc.subject	Vasoconstriction	en_US
dc.title	Store-operated channels in the pulmonary circulation of high- and low-altitude neonatal lambs	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1152/ajplung.00024.2012
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.01.08
dc.relation.issn	1522-1504