Universidad Peruana Cayetano Heredia

Genome sequence and comparative analysis of Avibacterium paragallinarum

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dc.contributor.author Requena, David
dc.contributor.author Chumbe, Ana
dc.contributor.author Torres, Michael
dc.contributor.author Alzamora, Ofelia
dc.contributor.author Ramirez, Manuel
dc.contributor.author Valdivia-Olarte, Hugo
dc.contributor.author Gutierrez, Andres Hazaet
dc.contributor.author Izquierdo-Lara, Ray
dc.contributor.author Saravia, Luis Enrique
dc.contributor.author Zavaleta, Milagros
dc.contributor.author Tataje-Lavanda, Luis
dc.contributor.author Best, Ivan
dc.contributor.author Fernández-Sánchez, Manolo
dc.contributor.author Icochea, Eliana
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Fernández-Díaz, Manolo
dc.date.accessioned 2022-01-04T20:29:54Z
dc.date.available 2022-01-04T20:29:54Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10384
dc.description.abstract Background: Avibacterium paragallinarum, the causative agent of infectious coryza, is a highly contagious respiratory acute disease of poultry, which affects commercial chickens, laying hens and broilers worldwide. Methodology: In this study, we performed the whole genome sequencing, assembly and annotation of a Peruvian isolate of A. paragallinarum. Genome was sequenced in a 454 GS FLX Titanium system. De novo assembly was performed and annotation was completed with GS De Novo Assembler 2.6 using the H. influenzae str. F3031 gene model. Manual curation of the genome was performed with Artemis. Putative function of genes was predicted with Blast2GO. Virulence factors were identified by comparison with the Virulence Factor Database. Results: The genome obtained has a length of 2.47 Mb with 40.66% of GC content. Seventy five large contigs (>500 nt) were obtained, which comprised 1,204 predicted genes. All the contigs are available in Genbank [GenBank: PRJNA64665]. A total of 103 virulence factors, reported in the Virulence Factor Database, were found in A. paragallinarum. Forty four of them are present in 7 species of Haemophilus, which are related with pathogenesis, virulence and host immune system evasion. A tetracycline-resistance associated transposon (Tn10), was found in A. paragallinarum, possibly acting as a defense mechanism. Discussion and conclusion: The availability of A. paragallinarum genome represents an important source of information for the development of diagnostic tests, genotyping, and novel antigens for potential vaccines against infectious coryza. Identification of virulence factors contributes to better understanding the pathogenesis, and planning efforts for prevention and control of the disease. en_US
dc.language.iso eng
dc.publisher Biomedical Informatics
dc.relation.ispartofseries Bioinformation
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Infectious coryza en_US
dc.subject genome sequencing en_US
dc.subject virulence factors en_US
dc.subject syntenic homology en_US
dc.subject Tn10 transposon en_US
dc.title Genome sequence and comparative analysis of Avibacterium paragallinarum en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.6026/97320630009528
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.00
dc.relation.issn 0973-2063


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