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Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogans serovar Copenhageni (M20) derived antigen

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dc.contributor.author Canal, Enrique
dc.contributor.author Pollett, Simon
dc.contributor.author Heitzinger, Kristen
dc.contributor.author Gregory, Michael
dc.contributor.author Kasper, Matthew
dc.contributor.author Halsey, Eric
dc.contributor.author Meza, Yocelinda
dc.contributor.author Campos, Kalina
dc.contributor.author Perez, Juan
dc.contributor.author Meza, Rina
dc.contributor.author Bernal, Maruja
dc.contributor.author Guillen, Alfredo
dc.contributor.author Kochel, Tadeusz J.
dc.contributor.author Espinosa, Benjamin
dc.contributor.author Hall, Eric R.
dc.contributor.author Maves, Ryan C.
dc.date.accessioned 2022-01-04T20:29:55Z
dc.date.available 2022-01-04T20:29:55Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10395
dc.description.abstract Background: Leptospirosis is a potentially lethal zoonosis mainly affecting low-resource tropical countries, including Peru and its neighbouring countries. Timely diagnosis of leptospirosis is critical but may be challenging in the regions where it is most prevalent. The serodiagnostic gold standard microagglutination test (MAT) may be technically prohibitive. Our Objetive: in this study was to assess the sensitivity, specificity, and predictive value of an IgM antibody capture enzyme-linked immunoassay (MAC-ELISA) derived from the M20 strain of Leptospira interrogans serovar Copenhageni (M20) by comparison to MAT, which was used as the gold standard of diagnosis. Methods: Acute and convalescent sera from participants participating in a passive febrile surveillance study in multiple regions of Peru were tested by both IgM MAC-ELISA and MAT. The sensitivity, specificity, positive and negative predictive value (PPV, NPV) of the MAC-ELISA assay for acute, convalescent and paired sera by comparison to MAT were calculated. Results: The sensitivity, specificity, PPV and NPV of the MAC-ELISA assay for acute sera were 92.3%, 56.0%, 35.3% and 96.6% respectively. For convalescent sera, the sensitivity, specificity, PPV and NPV of the MAC-ELISA assay were 93.3%, 51.5%, 63.6% and 89.5% respectively. For paired sera, the sensitivity, specificity, PPV and NPV of the MAC-ELISA assay were 93.6%, 37.5%, 59.2%, 85.7% respectively. Conclusions: The M20 MAC-ELISA assay performed with a high sensitivity and low specificity in the acute phase of illness. Sensitivity was similar as compared with MAT in the convalescent phase and specificity remained low. Paired sera were the most sensitive but least specific by comparison to MAT serodiagnosis. NPV for acute, convalescent and paired sera was high. The limited specificity and high sensitivity of the MAC-ELISA IgM suggests that it would be most valuable to exclude leptospirosis in low-resource regions that lack immediate access to definitive reference laboratory techniques such as MAT. en_US
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartofseries BMC Infectious Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Human en_US
dc.subject major clinical study en_US
dc.subject Peru en_US
dc.subject enzyme linked immunosorbent assay en_US
dc.subject sensitivity and specificity en_US
dc.subject bacterial strain en_US
dc.subject blood sampling en_US
dc.subject predictive value en_US
dc.subject receiver operating characteristic en_US
dc.subject bacterium detection en_US
dc.subject diagnostic test accuracy study en_US
dc.subject gold standard en_US
dc.subject intermethod comparison en_US
dc.subject fever en_US
dc.subject leptospirosis en_US
dc.subject Leptospirosis en_US
dc.subject Antibodies, Bacterial en_US
dc.subject Antigens, Bacterial en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject immunoglobulin M antibody en_US
dc.subject Leptospira interrogans en_US
dc.subject agglutination test en_US
dc.subject Fever en_US
dc.subject IgM en_US
dc.subject MAC ELISA en_US
dc.subject MAT en_US
dc.subject microagglutination test en_US
dc.title Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogans serovar Copenhageni (M20) derived antigen en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/1471-2334-13-438
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1471-2334

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