dc.contributor.author |
Barletta, Francesca |
|
dc.contributor.author |
Otero Vegas, Larissa |
|
dc.contributor.author |
Collantes, Jimena |
|
dc.contributor.author |
Asto, Belisa |
|
dc.contributor.author |
de Jong, Bouke C. |
|
dc.contributor.author |
Seas Ramos, Carlos Rafael |
|
dc.contributor.author |
Rigouts, Leen |
|
dc.date.accessioned |
2022-01-04T20:29:55Z |
|
dc.date.available |
2022-01-04T20:29:55Z |
|
dc.date.issued |
2013 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/10397 |
|
dc.description.abstract |
Background: The aim of this study was to investigate the genetic diversity among Mycobacterium tuberculosis complex circulating in patients with no known risk factors for multi-drug resistant (MDR) tuberculosis (TB) living in a high MDR burden area and analyze the relationship between genotypes, primary drug resistance and age. Methods: Samples were collected during January-July 2009. Isolates were tested for drug susceptibility to first-line drugs and were genotyped by spoligotyping and the 15-loci Mycobacterial Interspersed Repetitive Unit (MIRU15). Results: Among the 199 isolates analyzed, 169 (84.9%) were identified in the SpolDB4.0 and 30 (15.1%) could not be matched to any lineage. The most prevalent lineage was Haarlem (29.6%), followed by T (15.6%), Beijing (14.1%), Latin American Mediterranean (12.6%) and U (8.5%). A few isolates belonged to the X and S clades (4.5%). Spoligotype analysis identified clustering among 148 of 169 isolates, whereas with MIRU15 all isolates were unique. Out of 197 strains; 31.5% were resistant to at least one drug, 7.5% were MDR and 22.3% showed any resistance to isoniazid. Conclusion: In contrast with other Latin-American countries where LAM lineage is the most predominant, we found the spoligotype 50 from the Haarlem lineage as the most common. None of the prevailing lineages showed a significant association with age or resistance to isoniazid and/or rifampicin. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
BioMed Central |
|
dc.relation.ispartofseries |
BMC Infectious Diseases |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
Humans |
en_US |
dc.subject |
Middle Aged |
en_US |
dc.subject |
Peru |
en_US |
dc.subject |
Prospective Studies |
en_US |
dc.subject |
Microbial Sensitivity Tests |
en_US |
dc.subject |
Genotype |
en_US |
dc.subject |
Genetic Variation |
en_US |
dc.subject |
Antitubercular |
en_US |
dc.subject |
Mycobacterium tuberculosiseffects |
en_US |
dc.subject |
Tuberculosis |
en_US |
dc.title |
Genetic variability of Mycobacterium tuberculosis complex in patients with no known risk factors for MDR-TB in the North-eastern part of Lima, Peru |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.1186/1471-2334-13-397 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#3.03.08 |
|
dc.relation.issn |
1471-2334 |
|