DSpace Repository

Theoretical analysis of the neuraminidase epitope of the Mexican A H1N1 influenza strain, and experimental studies on its interaction with rabbit and human hosts

Show simple item record

dc.contributor.author Reyes Loyola, Paola Kinara
dc.contributor.author Campos-Rodríguez, R.
dc.contributor.author Bello, M.
dc.contributor.author Rojas-Hernández, S.
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Quiliano, Miguel
dc.contributor.author Briz, Verónica
dc.contributor.author Muñoz-Fernández, M.Angeles
dc.contributor.author Tolentino-López, Luis
dc.contributor.author Correa-Basurto, Jose
dc.date.accessioned 2022-01-04T20:29:59Z
dc.date.available 2022-01-04T20:29:59Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10501
dc.description.abstract The neuraminidase (NA) epitope from the Mexican AH1N1 influenza virus was identified by using sequences registered at the GenBank during the peak of a pandemic (from April 2009 to October 2010). First, NA protein sequences were submitted for multiple alignment analysis, and their three-dimensional models (3-D) were then built by using homology modeling. The most common sequence (denominated wild-type) and its mutants were submitted to linear and nonlinear epitope predictors, which included the major histocompatibility complex type II (MHC II) and B-cell peptides. The epitope prediction was in accordance with evolutionary behavior and some protein structural properties. The latter included a low NA mutation rate, NA 3-D surface exposure, and the presence of high hindrance side chain residues. After selecting the epitope, docking studies and molecular dynamics (MD) simulations were used to explore interactions between the epitope and MHC II. Afterward, several experimental assays were performed to validate the theoretical study by using antibodies from humans (infected by pandemic H1N1) and rabbits (epitope vaccination). The results show 119 complete sequences that were grouped into 28 protein sequences according to their identity (one wild-type and 27 representative mutants (1–5 mutations)). The predictors yielded several epitopes, with the best fit being the one located in the C-terminal region. Theoretical Methods: demonstrated that the selected epitope reached the P4, P6, P7, and P9 pockets of MHC II, whereas the experimental evidence indicates that the epitope is recognized by human antibodies and also by rabbit antibodies immunized with the peptide. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartofseries Immunologic Research
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Epitope vaccine en_US
dc.subject Neuraminidase en_US
dc.subject Prediction of immunogenic epitopes en_US
dc.subject Influenza AH1N1 en_US
dc.subject Docking en_US
dc.subject Molecular dynamics simulations en_US
dc.title Theoretical analysis of the neuraminidase epitope of the Mexican A H1N1 influenza strain, and experimental studies on its interaction with rabbit and human hosts en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s12026-013-8385-z
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.03
dc.relation.issn 1559-0755


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics