dc.contributor.author |
Rojas-Ponce, G |
|
dc.contributor.author |
Rachow, A |
|
dc.contributor.author |
Guerra, H |
|
dc.contributor.author |
Mapamba, D |
|
dc.contributor.author |
Joseph, J |
|
dc.contributor.author |
Mlundi, R |
|
dc.contributor.author |
Marimoto, S |
|
dc.contributor.author |
Ntinginya, NE |
|
dc.contributor.author |
Mangu, C |
|
dc.contributor.author |
Framhein, A |
|
dc.contributor.author |
Butler, A |
|
dc.contributor.author |
Kohlenberg, A |
|
dc.contributor.author |
Ngatemelela, D |
|
dc.contributor.author |
Froeschl, G |
|
dc.contributor.author |
Maboko, L |
|
dc.contributor.author |
Hoelscher, M |
|
dc.contributor.author |
Heinrich, N |
|
dc.date.accessioned |
2022-01-04T20:30:01Z |
|
dc.date.available |
2022-01-04T20:30:01Z |
|
dc.date.issued |
2013 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/10528 |
|
dc.description.abstract |
OBJECTIVE: To develop a new liquid culture Method: to detect Mycobacterium tuberculosis complex (MTC) in sputum using 2,3-diphenyl-5-thienyl-(2)-tetrazolium (STC), the nitrate reductase assay (NRA) and p-nitrobenzoic acid (PNB). DESIGN: Ninety-three sputum samples collected from 18 tuberculosis patients were decontaminated with N-acetyl-L-cysteine-sodium hydroxide using MGIT™ 960 and in STC-NRA cultures, both in the presence and in the absence of PNB, an inhibitor of MTC growth. The reduction of STC by colour change indicated mycobacterial growth; NRA was then performed to confirm MTC. RESULTS: STC-NRA culture was positive for acid-fast bacilli in 66/93 (71%) samples, of which 60/93 (64.5%) were identified as MTC-positive and 6/93 (6.5%) as indeterminate mycobacteria. MGIT indicated MTC in 59/93 (63.4%) cultures. Contamination was detected in 12/93 (13%) STC-NRA cultures vs. 29/93 (31.2%) MGIT cultures. The mean time to detection (TTD) of MTC using STC-NRA was 14 days and 7 days using MGIT. CONCLUSION: The STC-NRA Method: is sensitive for the detection of MTC in sputum. TTD increased with duration of anti-tuberculosis treatment, highlighting the value of this Method: in monitoring treatment success. The Method: is simple and inexpensive and, unlike MGIT, does not require technical equipment. The preliminary performance characteristics of the Method: should be further evaluated in larger studies. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
International Union Against Tuberculosis and Lung Disease |
|
dc.relation.ispartofseries |
International Journal of Tuberculosis and Lung Disease |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
2-3-diphenyl-5-thienyl-(2)-tetrazolium STC |
en_US |
dc.subject |
TTD |
en_US |
dc.subject |
liquid culture |
en_US |
dc.subject |
nitrate reductase assay |
en_US |
dc.subject |
treatment monitoring |
en_US |
dc.title |
A continuously monitored colorimetric method for detection of Mycobacterium tuberculosis complex in sputum |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.5588/ijtld.13.0317 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#3.03.08 |
|
dc.relation.issn |
1815-7920 |
|