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dc.contributor.author | Rojas-Ponce, G | |
dc.contributor.author | Rachow, A | |
dc.contributor.author | Guerra, H | |
dc.contributor.author | Mapamba, D | |
dc.contributor.author | Joseph, J | |
dc.contributor.author | Mlundi, R | |
dc.contributor.author | Marimoto, S | |
dc.contributor.author | Ntinginya, NE | |
dc.contributor.author | Mangu, C | |
dc.contributor.author | Framhein, A | |
dc.contributor.author | Butler, A | |
dc.contributor.author | Kohlenberg, A | |
dc.contributor.author | Ngatemelela, D | |
dc.contributor.author | Froeschl, G | |
dc.contributor.author | Maboko, L | |
dc.contributor.author | Hoelscher, M | |
dc.contributor.author | Heinrich, N | |
dc.date.accessioned | 2022-01-04T20:30:01Z | |
dc.date.available | 2022-01-04T20:30:01Z | |
dc.date.issued | 2013 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12866/10528 | |
dc.description.abstract | OBJECTIVE: To develop a new liquid culture Method: to detect Mycobacterium tuberculosis complex (MTC) in sputum using 2,3-diphenyl-5-thienyl-(2)-tetrazolium (STC), the nitrate reductase assay (NRA) and p-nitrobenzoic acid (PNB). DESIGN: Ninety-three sputum samples collected from 18 tuberculosis patients were decontaminated with N-acetyl-L-cysteine-sodium hydroxide using MGIT™ 960 and in STC-NRA cultures, both in the presence and in the absence of PNB, an inhibitor of MTC growth. The reduction of STC by colour change indicated mycobacterial growth; NRA was then performed to confirm MTC. RESULTS: STC-NRA culture was positive for acid-fast bacilli in 66/93 (71%) samples, of which 60/93 (64.5%) were identified as MTC-positive and 6/93 (6.5%) as indeterminate mycobacteria. MGIT indicated MTC in 59/93 (63.4%) cultures. Contamination was detected in 12/93 (13%) STC-NRA cultures vs. 29/93 (31.2%) MGIT cultures. The mean time to detection (TTD) of MTC using STC-NRA was 14 days and 7 days using MGIT. CONCLUSION: The STC-NRA Method: is sensitive for the detection of MTC in sputum. TTD increased with duration of anti-tuberculosis treatment, highlighting the value of this Method: in monitoring treatment success. The Method: is simple and inexpensive and, unlike MGIT, does not require technical equipment. The preliminary performance characteristics of the Method: should be further evaluated in larger studies. | en_US |
dc.language.iso | eng | |
dc.publisher | International Union Against Tuberculosis and Lung Disease | |
dc.relation.ispartofseries | International Journal of Tuberculosis and Lung Disease | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
dc.subject | 2-3-diphenyl-5-thienyl-(2)-tetrazolium STC | en_US |
dc.subject | TTD | en_US |
dc.subject | liquid culture | en_US |
dc.subject | nitrate reductase assay | en_US |
dc.subject | treatment monitoring | en_US |
dc.title | A continuously monitored colorimetric method for detection of Mycobacterium tuberculosis complex in sputum | en_US |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | https://doi.org/10.5588/ijtld.13.0317 | |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.03.08 | |
dc.relation.issn | 1815-7920 |
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