Universidad Peruana Cayetano Heredia

A continuously monitored colorimetric method for detection of Mycobacterium tuberculosis complex in sputum

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dc.contributor.author Rojas-Ponce, G
dc.contributor.author Rachow, A
dc.contributor.author Guerra, H
dc.contributor.author Mapamba, D
dc.contributor.author Joseph, J
dc.contributor.author Mlundi, R
dc.contributor.author Marimoto, S
dc.contributor.author Ntinginya, NE
dc.contributor.author Mangu, C
dc.contributor.author Framhein, A
dc.contributor.author Butler, A
dc.contributor.author Kohlenberg, A
dc.contributor.author Ngatemelela, D
dc.contributor.author Froeschl, G
dc.contributor.author Maboko, L
dc.contributor.author Hoelscher, M
dc.contributor.author Heinrich, N
dc.date.accessioned 2022-01-04T20:30:01Z
dc.date.available 2022-01-04T20:30:01Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10528
dc.description.abstract OBJECTIVE: To develop a new liquid culture Method: to detect Mycobacterium tuberculosis complex (MTC) in sputum using 2,3-diphenyl-5-thienyl-(2)-tetrazolium (STC), the nitrate reductase assay (NRA) and p-nitrobenzoic acid (PNB). DESIGN: Ninety-three sputum samples collected from 18 tuberculosis patients were decontaminated with N-acetyl-L-cysteine-sodium hydroxide using MGIT™ 960 and in STC-NRA cultures, both in the presence and in the absence of PNB, an inhibitor of MTC growth. The reduction of STC by colour change indicated mycobacterial growth; NRA was then performed to confirm MTC. RESULTS: STC-NRA culture was positive for acid-fast bacilli in 66/93 (71%) samples, of which 60/93 (64.5%) were identified as MTC-positive and 6/93 (6.5%) as indeterminate mycobacteria. MGIT indicated MTC in 59/93 (63.4%) cultures. Contamination was detected in 12/93 (13%) STC-NRA cultures vs. 29/93 (31.2%) MGIT cultures. The mean time to detection (TTD) of MTC using STC-NRA was 14 days and 7 days using MGIT. CONCLUSION: The STC-NRA Method: is sensitive for the detection of MTC in sputum. TTD increased with duration of anti-tuberculosis treatment, highlighting the value of this Method: in monitoring treatment success. The Method: is simple and inexpensive and, unlike MGIT, does not require technical equipment. The preliminary performance characteristics of the Method: should be further evaluated in larger studies. en_US
dc.language.iso eng
dc.publisher International Union Against Tuberculosis and Lung Disease
dc.relation.ispartofseries International Journal of Tuberculosis and Lung Disease
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject 2-3-diphenyl-5-thienyl-(2)-tetrazolium STC en_US
dc.subject TTD en_US
dc.subject liquid culture en_US
dc.subject nitrate reductase assay en_US
dc.subject treatment monitoring en_US
dc.title A continuously monitored colorimetric method for detection of Mycobacterium tuberculosis complex in sputum en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.5588/ijtld.13.0317
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1815-7920


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