Universidad Peruana Cayetano Heredia

In vitro development and analysis of escherichia coli and shigella boydii azithromycin-resistant mutants

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dc.contributor.author Gomes, Claudia
dc.contributor.author Pons, Maria J.
dc.contributor.author Magallon-Tejada, Ariel
dc.contributor.author Durand Vara, David Percy
dc.contributor.author Lluque, Angela
dc.contributor.author Mosquito, Susan
dc.contributor.author Riveros Ramirez, Maribel Denise
dc.contributor.author Mercado, Erik
dc.contributor.author Prada, Ana
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Ruiz, Joaquim
dc.date.accessioned 2022-01-04T20:31:45Z
dc.date.available 2022-01-04T20:31:45Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10608
dc.description.abstract Three clinical isolates of E. coli and one S. boydii isolated from feces samples collected from children under 5 years of age with diarrhea in Lima, Peru were inoculated onto Mueller-Hinton plates containing increasing serial dilutions of AZM ranging from their specific minimal inhibitory concentration (2 or 4 mg/l) to 64 mg/l. From these plates, 16 AZM-resistant mutants were selected to determine the stability of the resistance and the presence of cross resistance with other antibiotics. The role of Phe-Arg-β-Naphthylamide (PAβN)-inhibitible efflux pumps as well as the presence of mutations in the rplV, rplD, and rrlH (23S rRNA) genes and alterations in the outer membrane profiles were determined in these 16 mutants. The rate of mutation ranged from < 2.70×10−10 to 2.17×10−7 for E. coli and from < 9.58×10−10 to 1.05×10−8 for S. boydii. E. coli mutants showed an increase in the AZM-MIC up to sixfold with one strain achieving a MIC >256 mg/l. In contrast, S. boydii only presented increases of up to twofold in MIC levels. All the strains obtained, but one showed stable AZM resistance. In the presence of PAβN, the AZM MICs decreased to parental levels in Shigella mutants, while no MIC returned to parental levels among the E. coli mutants. No cross resistance to other classes of antibiotics was found. These results show the relevance of PAβN-inhibitible efflux pumps in the basal levels and development of AZM resistance. Further studies to characterize the remaining unidentified mechanisms of AZM resistance are needed. en_US
dc.language.iso eng
dc.publisher Mary Ann Liebert
dc.relation.ispartofseries Microbial Drug Resistance
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject controlled study en_US
dc.subject priority journal en_US
dc.subject in vitro study en_US
dc.subject Microbial Sensitivity Tests en_US
dc.subject Membrane Transport Proteins en_US
dc.subject article en_US
dc.subject Anti-Bacterial Agents en_US
dc.subject Escherichia coli en_US
dc.subject minimum inhibitory concentration en_US
dc.subject antibiotic resistance en_US
dc.subject Drug Resistance, Bacterial en_US
dc.subject Dose-Response Relationship, Drug en_US
dc.subject Mutation en_US
dc.subject Dipeptides en_US
dc.subject Escherichia coli Infections en_US
dc.subject azithromycin en_US
dc.subject Azithromycin en_US
dc.subject cross resistance en_US
dc.subject Shigella en_US
dc.subject Shigella boydii en_US
dc.subject Dysentery, Bacillary en_US
dc.subject mutation en_US
dc.subject outer membrane en_US
dc.title In vitro development and analysis of escherichia coli and shigella boydii azithromycin-resistant mutants en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1089/mdr.2012.0036
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1931-8448


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