Universidad Peruana Cayetano Heredia

Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity

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dc.contributor.author Arana, Yanina
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Tuero Ochoa, Iskra
dc.contributor.author Grandjean, Louis
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Gilman, Robert Hugh
dc.date.accessioned 2022-01-04T20:31:46Z
dc.date.available 2022-01-04T20:31:46Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10632
dc.description.abstract This study examines the carbohydrate composition of Taenia solium whole oncosphere antigens (WOAs), in order to improve the understanding of the antigenicity of the T. solium. Better knowledge of oncosphere antigens is crucial to accurately diagnose previous exposure to T. solium eggs and thus predict the development of neurocysticercosis. A set of seven lectins conjugates with wide carbohydrate specificity were used on parasite fixations and somatic extracts. Lectin fluorescence revealed that D-mannose, D-glucose, D-galactose and N-acetyl-D-galactosamine residues were the most abundant constituents of carbohydrate chains on the surface of T. solium oncosphere. Lectin blotting showed that posttranslational modification with N-glycosylation was abundant while little evidence of O-linked carbohydrates was observed. Chemical oxidation and enzymatic deglycosylation in situ were performed to investigate the immunoreactivity of the carbohydrate moieties. Linearizing or removing the carbohydrate moieties from the protein backbones did not diminish the immunoreactivity of these antigens, suggesting that a substantial part of the host immune response against T. solium oncosphere is directed against the peptide epitopes on the parasite antigens. Finally, using carbohydrate probes, we demonstrated for the first time that the presence of several lectins on the surface of the oncosphere was specific to carbohydrates found in intestinal mucus, suggesting a possible role in initial attachment of the parasite to host cells. en_US
dc.language.iso eng
dc.publisher Springer Verlag
dc.relation.ispartofseries Parasitology Research
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Sialic Acid en_US
dc.subject Wheat Germ Agglutinin en_US
dc.subject Cysticercosis en_US
dc.subject Sodium en_US
dc.subject Periodate en_US
dc.subject Echinococcus Granulosus en_US
dc.title Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s00436-013-3542-9
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1432-1955


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