DSpace Repository

Detection of secretory immunoglobulin a in human colostrum as mucosal immune response against proteins of the type III secretion system of Salmonella, Shigella and enteropathogenic Escherichia Coli

Show simple item record

dc.contributor.author Durand Vara, David Percy
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Bellomo, Sicilia M. E.
dc.contributor.author Contreras, Carmen A.
dc.contributor.author Bustamante, Víctor H.
dc.contributor.author Ruiz, Joaquim
dc.contributor.author Cleary, Thomas G.
dc.date.accessioned 2022-01-04T20:31:46Z
dc.date.available 2022-01-04T20:31:46Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10638
dc.description.abstract Some enteropathogens use the type III secretion system to secrete proteins that allows them to interact with enterocytes and promote bacterial attachment or intracellular survival. These proteins are Salmonella invasion proteins (Sip), invasion plasmid antigens (Ipa) of Shigella and Escherichia coli secreted proteins (Esp) of enteropathogenic E. coli. There are no previous studies defining the presence of colostral sIgA against all these 3 major enteric pathogens. Objective: To evaluate the presence of sIgA in colostrum against proteins of the type III secretion system of Salmonella, Shigella and enteropathogenic E. coli. Methods: We collected 76 colostrum samples from puerperal women in Lima, Peru. These samples were reacted with type III secretion system proteins extracted from bacterial culture supernatants and evaluated by Western Blot. Results: Antibodies were detected against Salmonella antigens SipA in 75 samples (99%), SipC in 62 (82%) and SipB in 31 (41%); against Shigella antigens IpaC in 70 (92%), IpaB in 68 (89%), IpaA in 66 (87%) and IpaD in 41 (54%); and against enteropathogenic E. coli EspC in 70 (92%), EspB-D in 65 (86%) and EspA in 41 (54%). Ten percent of samples had antibodies against all proteins evaluated and 42% against all except 1 protein. There was no sample negative to all these proteins. Conclusions: The extraordinarily high frequency of antibodies in colostrum of puerperal women detected in this study against these multiple enteric pathogens shows evidence of immunological memory and prior exposure to these pathogens, in addition to its possible protective role against infection. en_US
dc.language.iso eng
dc.publisher Wolters Kluwer Health
dc.relation.ispartofseries Pediatric Infectious Disease Journal
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Blotting, Western en_US
dc.subject Antibodies, Bacterial en_US
dc.subject Bacterial Proteins en_US
dc.subject Colostrum en_US
dc.subject Enteropathogenic Escherichia coli en_US
dc.subject Immunoglobulin A, Secretory en_US
dc.subject Shigella en_US
dc.title Detection of secretory immunoglobulin a in human colostrum as mucosal immune response against proteins of the type III secretion system of Salmonella, Shigella and enteropathogenic Escherichia Coli en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1097/INF.0b013e318293306c
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.03
dc.relation.issn 1532-0987


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics