DSpace Repository

A FRET-Based Real-Time PCR Assay to Identify the Main Causal Agents of New World Tegumentary Leishmaniasis

Show simple item record

dc.contributor.author Tsukayama Cisneros, Pablo
dc.contributor.author Núñez, Jorge H.
dc.contributor.author De Los Santos, Maxy
dc.contributor.author Soberón, Valeria
dc.contributor.author Lucas, Carmen M.
dc.contributor.author Matlashewski, Greg
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Ore, Marianela
dc.contributor.author Baldeviano, G.Christian
dc.contributor.author Edgel, Kimberly A.
dc.contributor.author Lescano Guevara, Andres Guillermo
dc.contributor.author Graf, Paul C.F.
dc.contributor.author Bacon, David J.
dc.date.accessioned 2022-01-04T20:31:47Z
dc.date.available 2022-01-04T20:31:47Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10661
dc.description.abstract In South America, various species of Leishmania are endemic and cause New World tegumentary leishmaniasis (NWTL). The correct identification of these species is critical for adequate clinical management and surveillance activities. We developed a real-time polymerase chain reaction (PCR) assay and evaluated its diagnostic performance using 64 archived parasite isolates and 192 prospectively identified samples collected from individuals with suspected leishmaniasis enrolled at two reference clinics in Lima, Peru. The real-time PCR assay was able to detect a single parasite and provided unambiguous melting peaks for five Leishmania species of the Viannia subgenus that are highly prevalent in South America: L. (V.) braziliensis, L. (V.) panamensis, L. (V.) guyanensis, L. (V.) peruviana and L. (V.) lainsoni. Using kinetoplastid DNA-based PCR as a gold standard, the real-time PCR had sensitivity and specificity values of 92% and 77%, respectively, which were significantly higher than those of conventional tests such as microscopy, culture and the leishmanin skin test (LST). In addition, the real-time PCR identified 147 different clinical samples at the species level, providing an overall agreement of 100% when compared to multilocus sequence typing (MLST) data performed on a subset of these samples. Furthermore, the real-time PCR was three times faster and five times less expensive when compared to PCR - MLST for species identification from clinical specimens. In summary, this new assay represents a cost-effective and reliable alternative for the identification of the main species causing NWTL in South America. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject South America en_US
dc.subject human en_US
dc.subject major clinical study en_US
dc.subject microscopy en_US
dc.subject species identification en_US
dc.subject sensitivity and specificity en_US
dc.subject real time polymerase chain reaction en_US
dc.subject gold standard en_US
dc.subject article en_US
dc.subject cost benefit analysis en_US
dc.subject leishmaniasis en_US
dc.subject multilocus sequence typing en_US
dc.subject cross reaction en_US
dc.subject leishmanin skin test en_US
dc.subject DNA isolation en_US
dc.subject fluorescence resonance energy transfer en_US
dc.subject high resolution melting analysis en_US
dc.subject tegumentary leishmaniasis en_US
dc.title A FRET-Based Real-Time PCR Assay to Identify the Main Causal Agents of New World Tegumentary Leishmaniasis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0001956
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics