Universidad Peruana Cayetano Heredia

Bovine Lactoferrin Decreases Cholera-Toxin-Induced Intestinal Fluid Accumulation in Mice by Ganglioside Interaction

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dc.contributor.author Rivera, Fulton P.
dc.contributor.author Medina, Anicia M.
dc.contributor.author Bezada, Sandra
dc.contributor.author Valencia, Roberto
dc.contributor.author Bernal, María
dc.contributor.author Meza, Rina
dc.contributor.author Maves, Ryan C.
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.date.accessioned 2022-01-04T20:31:49Z
dc.date.available 2022-01-04T20:31:49Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10697
dc.description.abstract Secretory diarrhea caused by cholera toxin (CT) is initiated by binding of CT’s B subunit (CTB) to GM1-ganglioside on the surface of intestinal cells. Lactoferrin, a breast milk glycoprotein, has shown protective effect against several enteropathogens. The aims of this study were to determine the effect of bovine-lactoferrin (bLF) on CT-induced intestinal fluid accumulation in mice, and the interaction between bLF and CT/CTB with the GM1-ganglioside receptor. Fluid accumulation induced by CT was evaluated in the mouse ileal loop model using 56 BALB/c mice, with and without bLF added before, after or at the same time of CT administration. The effect of bLF in the interaction of CT and CTB with GM1-ganglioside was evaluated by a GM1-enzyme-linked immunosorbent assay. bLF decreased CT-induced fluid accumulation in the ileal loop of mice. The greatest effect was when bLF was added before CT (median, 0.066 vs. 0.166 g/cm, with and without bLF respectively, p<0.01). We conclude that bLF decreases binding of CT and CTB to GM1-ganglioside, suggesting that bLF suppresses CT-induced fluid accumulation by blocking the binding of CTB to GM1-ganglioside. bLF may be effective as adjunctive therapy for treatment of cholera diarrhea. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Mice en_US
dc.subject Cattle en_US
dc.subject Dose-Response Relationship, Drug en_US
dc.subject Chlorides en_US
dc.subject Cholera Toxin en_US
dc.subject Enterotoxins en_US
dc.subject Escherichia coli en_US
dc.subject Ferric Compounds en_US
dc.subject G(M1) Ganglioside en_US
dc.subject Gangliosides en_US
dc.subject Intestinal Mucosa en_US
dc.subject Intestines en_US
dc.subject Lactoferrin en_US
dc.subject Protein Binding en_US
dc.subject Receptors Cell Surface en_US
dc.title Bovine Lactoferrin Decreases Cholera-Toxin-Induced Intestinal Fluid Accumulation in Mice by Ganglioside Interaction en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0059253
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.11
dc.relation.issn 1932-6203


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