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Detection of Soluble Antigen and DNA of Trypanosoma cruzi in Urine Is Independent of Renal Injury in the Guinea Pig Model

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dc.contributor.author Castro-Sesquen, Yagahira E.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Yauri, Verónica
dc.contributor.author Cok, Jaime
dc.contributor.author Angulo, Noelia
dc.contributor.author Escalante, Hermes
dc.contributor.author Bern, Caryn
dc.date.accessioned 2022-01-04T20:31:49Z
dc.date.available 2022-01-04T20:31:49Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10700
dc.description.abstract The diagnosis of Chagas disease in humans is generally limited to the detection of specific antibodies. Detection of T. cruzi antigens in urine has been reported previously, but is not used in the diagnosis. In this study, soluble T. cruzi antigens and DNA were detected in urine samples and were associated with kidney injury and systemic detection of the parasite. We used 72 guinea pigs infected with T. cruzi Y strain and 18 non-infected guinea pigs. Blood, kidney, heart and urine samples were collected during the acute phase and chronic phase. Urine samples were concentrated by ultrafiltration. Antigens were detected by Western Blot using a polyclonal antibody against trypomastigote excretory-secretory antigen (TESA). T. cruzi DNA was detected by PCR using primers 121/122 and TcZ1/TcZ2. Levels of T. cruzi DNA in blood, heart and kidney were determined by quantitative PCR. T. cruzi antigens (75 kDa, 80 kDa, 120 kDa, 150 kDa) were detected in the acute phase (67.5%) and the chronic phase (45%). Parasite DNA in urine was detected only in the acute phase (45%). Kidney injury was characterized by high levels of proteinuria, kidney injury molecule-1 (KIM-1) and urea, and some histopathological changes such as inflammation, necrosis, fibrosis and scarce parasites. The detection of antigens and DNA in urine was associated with the presence of parasite DNA in blood and heart and with high levels of parasite DNA in blood, but not with the presence of parasite in kidney or kidney injury. These results suggest that the detection of T. cruzi in urine could be improved to be a valuable Method: for the diagnosis of Chagas disease, particularly in congenital Chagas disease and in immunocompromised patients. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Antigens, Protozoan en_US
dc.subject Guinea Pigs en_US
dc.subject Trypanosoma cruzi en_US
dc.subject Chagas Disease en_US
dc.subject DNA, Protozoan en_US
dc.subject Hear en_US
dc.subject Kidney Diseases en_US
dc.subject Kidney en_US
dc.subject Membrane Glycoproteins en_US
dc.subject Polymerase Chain Reaction en_US
dc.title Detection of Soluble Antigen and DNA of Trypanosoma cruzi in Urine Is Independent of Renal Injury in the Guinea Pig Model en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0058480
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.27
dc.relation.issn 1932-6203


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