Dried blood spots for qPCR diagnosis of acute Bartonella bacilliformis infection

Torres, Lorena L.; Moore, David Alexander James; Mabey, David; Garcia Funegra, Patricia Jannet; Peeling, Rosanna W.; Smit, Pieter W.; Pérez-Lu, José E.

dc.contributor.author	Smit, Pieter W.
dc.contributor.author	Peeling, Rosanna W.
dc.contributor.author	Garcia Funegra, Patricia Jannet
dc.contributor.author	Torres, Lorena L.
dc.contributor.author	Pérez-Lu, José E.
dc.contributor.author	Moore, David Alexander James
dc.contributor.author	Mabey, David
dc.date.accessioned	2022-01-04T20:33:20Z
dc.date.available	2022-01-04T20:33:20Z
dc.date.issued	2013
dc.identifier.uri	https://hdl.handle.net/20.500.12866/10753
dc.description.abstract	Bartonella bacilliformis is the etiological agent of a life-threatening illness. Thin blood smear is the most common diagnostic Method: for acute infection in endemic areas of Peru but remains of limited value because of low sensitivity. The aim of this study was to adapt a B. bacilliformis-specific real-time polymerase chain reaction (PCR) assay for use with dried blood spots (DBS) as a sampling Method: and assess its performance and use for the diagnosis and surveillance of acute Bartonella infection. Only two of 65 children (3%) that participated in this study had positive blood smears for B. bacilliformis, whereas 16 (including these two) were positive by PCR performed on DBS samples (24.6%). The use of DBS in combination with B. bacilliformis-specific PCR could be a useful tool for public health in identifying and monitoring outbreaks of infection and designing control programs to reduce the burden of this life-threatening illness.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Humans	en_US
dc.subject	Real-Time Polymerase Chain Reaction	en_US
dc.subject	Bacteriological Techniques	en_US
dc.subject	Acute Disease	en_US
dc.subject	Dried Blood Spot Testing	en_US
dc.subject	Peru	en_US
dc.subject	Bacteremia	en_US
dc.subject	Bartonella bacilliformis	en_US
dc.subject	Bartonella Infections	en_US
dc.subject	DNA	en_US
dc.title	Dried blood spots for qPCR diagnosis of acute Bartonella bacilliformis infection	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.13-0246
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645