dc.contributor.author |
Battaglioli, T. |
|
dc.contributor.author |
Soto, A. |
|
dc.contributor.author |
Agapito, J. |
|
dc.contributor.author |
Acurio, V. |
|
dc.contributor.author |
Van der Stuyft, P. |
|
dc.date.accessioned |
2022-01-04T20:33:21Z |
|
dc.date.available |
2022-01-04T20:33:21Z |
|
dc.date.issued |
2014 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/10787 |
|
dc.description.abstract |
Objectives: To compare the performance of liquid culture on simple Middlebrook 7H9 to the one of manual mycobacterial growth indicator tube (MGIT) and solid culture on Ogawa for the diagnosis of smear-negative tuberculosis (SN-TB) in a high-burden, resource-constrained setting. Methods: Sputum samples from patients with clinical suspicion of SN-PTB admitted to two-third-level hospitals in Lima between September 2005 and May 2008 were cultured in parallel on simple Middlebrook 7H9, manual MGIT and Ogawa. A case of SN-TB was defined as one with a positive culture in any medium. Results: Among samples from 542 patients, 151 (28%) cases of SN-TB were identified. The sensitivity of Middlebrook 7H9 (0.76, 95% CI 0.69–0.83) was not substantially different from that of MGIT (0.85, 95% CI 0.79–0.91). Ogawa had the lowest sensitivity (0.63, 95% CI 0.55–0.71). The median turnaround time was similar for both liquid media (18 days), and it was shorter than that of Ogawa (30 days). Conclusions: Culture on simple Middlebrook 7H9 performs almost as well as MGIT, at a probably more affordable cost. Further studies on the cost-effectiveness of this overlooked technique should be performed. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
Wiley |
|
dc.relation.ispartofseries |
Tropical Medicine and International Health |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
Middlebrook 7H9 |
en_US |
dc.subject |
mycobacterial growth indicator tube |
en_US |
dc.subject |
Ogawa |
en_US |
dc.subject |
Mycobacterium tuberculosis |
en_US |
dc.subject |
diagnosis |
en_US |
dc.subject |
smear-negative tuberculosis |
en_US |
dc.title |
Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.1111/tmi.12384 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#3.03.06 |
|
dc.relation.issn |
1365-3156 |
|