Universidad Peruana Cayetano Heredia

Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis

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dc.contributor.author Battaglioli, T.
dc.contributor.author Soto, A.
dc.contributor.author Agapito, J.
dc.contributor.author Acurio, V.
dc.contributor.author Van der Stuyft, P.
dc.date.accessioned 2022-01-04T20:33:21Z
dc.date.available 2022-01-04T20:33:21Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/10787
dc.description.abstract Objectives: To compare the performance of liquid culture on simple Middlebrook 7H9 to the one of manual mycobacterial growth indicator tube (MGIT) and solid culture on Ogawa for the diagnosis of smear-negative tuberculosis (SN-TB) in a high-burden, resource-constrained setting. Methods: Sputum samples from patients with clinical suspicion of SN-PTB admitted to two-third-level hospitals in Lima between September 2005 and May 2008 were cultured in parallel on simple Middlebrook 7H9, manual MGIT and Ogawa. A case of SN-TB was defined as one with a positive culture in any medium. Results: Among samples from 542 patients, 151 (28%) cases of SN-TB were identified. The sensitivity of Middlebrook 7H9 (0.76, 95% CI 0.69–0.83) was not substantially different from that of MGIT (0.85, 95% CI 0.79–0.91). Ogawa had the lowest sensitivity (0.63, 95% CI 0.55–0.71). The median turnaround time was similar for both liquid media (18 days), and it was shorter than that of Ogawa (30 days). Conclusions: Culture on simple Middlebrook 7H9 performs almost as well as MGIT, at a probably more affordable cost. Further studies on the cost-effectiveness of this overlooked technique should be performed. en_US
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartofseries Tropical Medicine and International Health
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Middlebrook 7H9 en_US
dc.subject mycobacterial growth indicator tube en_US
dc.subject Ogawa en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject diagnosis en_US
dc.subject smear-negative tuberculosis en_US
dc.title Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/tmi.12384
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1365-3156


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