Universidad Peruana Cayetano Heredia

Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World

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dc.contributor.author Fraga, J.
dc.contributor.author Veland, N.
dc.contributor.author Montalvo, A.M.
dc.contributor.author Praet, N.
dc.contributor.author Boggild, A.K.
dc.contributor.author Valencia, B.M.
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Dujardin, J.-C.
dc.contributor.author Van der Auwera, G.
dc.date.accessioned 2022-01-18T19:26:46Z
dc.date.available 2022-01-18T19:26:46Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10849
dc.description.abstract The heat-shock protein 70 gene (hsp70) has been exploited for Leishmania species identification in the Old and New World, using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis. Three new Leishmania-specific hsp70 PCRs were recently described, and we applied 2 of these on 89 clinical samples from a total of 73 Peruvian patients with either cutaneous or mucocutaneous leishmaniasis. The new PCRs on average showed a 2- to 3-fold improved sensitivity in the tested sample types (lesion biopsies, aspirates, and scrapings), for both genus detection and species typing, and were most successful in biopsies. Leishmania braziliensis, L. peruviana, and L. guyanensis were encountered. About one third of the L. braziliensis parasites contained 2 hsp70 alleles. This study is a paradigm for the implementation of a globally applicable upgraded tool for the identification of Leishmania directly on human specimens from cutaneous and mucocutaneous lesions in the New World. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Diagnostic Microbiology and Infectious Disease
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject Controlled Study|Genotype en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject Allele en_US
dc.subject Leishmania Braziliensis en_US
dc.subject Leishmaniasis en_US
dc.subject Leishmaniasis Cutaneous en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject Skin Biopsy en_US
dc.subject Skin Leishmaniasis en_US
dc.subject Diagnostic Value en_US
dc.subject Limit Of Detection en_US
dc.subject Sensitivity And Specificity en_US
dc.subject Protozoan Proteins en_US
dc.subject Restriction Fragment Length Polymorphism en_US
dc.subject Leishmania en_US
dc.subject Intermethod Comparison en_US
dc.subject Heat Shock Protein 70 en_US
dc.subject Leishmania Peruviana en_US
dc.subject Species Identification en_US
dc.subject Accuracy en_US
dc.subject Genus en_US
dc.subject Hsp70 en_US
dc.subject HSP70 Heat-Shock Proteins en_US
dc.subject Identification en_US
dc.subject PCR-RFLP en_US
dc.title Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.diagmicrobio.2012.06.010
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1879-0070


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