Universidad Peruana Cayetano Heredia

Leishmania amazonensis META2 protein confers protection against heat shock and oxidative stress

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dc.contributor.author Ramos, C.S.
dc.contributor.author Yokoyama-Yasunaka, J.K.U.
dc.contributor.author Guerra-Giraldez, C.
dc.contributor.author Price, H.P.
dc.contributor.author Mortara, R.A.
dc.contributor.author Smith, D.F.
dc.contributor.author Uliana, S.R.B.
dc.date.accessioned 2022-01-18T19:26:46Z
dc.date.available 2022-01-18T19:26:46Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10852
dc.description.abstract The META cluster of Leishmania amazonensis contains both META1 and META2 genes, which are upregulated in metacyclic promastigotes and encode proteins containing the META domain. Previous studies defined META2 as a 48.0-kDa protein, which is conserved in other Leishmania species and in Trypanosoma brucei. In this work, we demonstrate that META2 protein expression is regulated during the Leishmania life cycle but constitutive in T. brucei. META2 protein is present in the cytoplasm and flagellum of L. amazonensis promastigotes. Leishmania META2-null replacement mutants are more sensitive to oxidative stress and, upon heat shock, assume rounded morphology with shortened flagella. The increased susceptibility of null parasites to heat shock is reversed by extra-chromosomal expression of the META2 gene. Defective Leishmania promastigotes exhibit decreased ability to survive in macrophages. By contrast, META2 expression is decreased by 80% in RNAi-induced T. brucei bloodstream forms with no measurable effect on survival or resistance to heat shock. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Experimental Parasitology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Controlled Study en_US
dc.subject Animal Cell en_US
dc.subject Animal Experiment en_US
dc.subject Leishmaniasis Cutaneous en_US
dc.subject Gene Expression Regulation en_US
dc.subject Macrophage en_US
dc.subject Protozoan Proteins en_US
dc.subject Leishmania en_US
dc.subject Mice Inbred BALB C en_US
dc.subject Antiprotozoal Agents en_US
dc.subject Leishmania Amazonensis en_US
dc.subject Leishmania Mexicana en_US
dc.subject Oxidative Stress en_US
dc.subject Fluorescent Antibody Technique en_US
dc.subject Heat Shock en_US
dc.subject Hot Temperature en_US
dc.subject Meglumine en_US
dc.subject META2 Gene en_US
dc.subject Metacyclogenesis en_US
dc.subject Microscopy Confocal en_US
dc.subject Mutant en_US
dc.subject Novobiocin en_US
dc.subject Nucleic Acid Synthesis Inhibitors en_US
dc.subject Organometallic Compounds en_US
dc.subject Parasite Survival en_US
dc.subject Promastigote en_US
dc.subject Trypanosoma Brucei en_US
dc.title Leishmania amazonensis META2 protein confers protection against heat shock and oxidative stress en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.exppara.2010.08.004
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1090-2449


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