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Molecular mechanisms of antibiotic resistance in diarrhoeagenic Escherichia coli isolated from children

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dc.contributor.author Mosquito, S.
dc.contributor.author Ruiz, Joaquim
dc.contributor.author Pons, M.J.
dc.contributor.author Durand Vara, David Percy
dc.contributor.author Barletta, F.
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.date.accessioned 2022-01-18T19:26:46Z
dc.date.available 2022-01-18T19:26:46Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10860
dc.description.abstract Diarrhoeagenic Escherichia coli (DEC) are an important cause of diarrhoea in children and are associated with high antibiotic resistance. However, there are few studies on the molecular mechanisms of resistance in this group of bacteria. The aim of this study was to determine the mechanisms associated with antibiotic resistance in the most common phenotypes of DEC. A total of 369 E. coli strains [commensal strains and DEC from children with (‘DEC-diarrhoea’) or without (‘DEC-control’) diarrhoea] isolated from children aged <1 year in periurban districts of Lima, Peru, were analysed. In total, 154 ampicillin-resistant strains (36 commensals, 33 DEC-control and 85 DEC-diarrhoea) were studied by PCR for the most prevalent resistance mechanisms to ampicillin, trimethoprim/sulfamethoxazole (SXT), tetracycline and chloramphenicol as well as for integrase types 1 and 2. In addition, restriction fragment length polymorphism was performed for SXT-resistant strains. Commensal strains were more frequently resistant to nalidixic acid and ciprofloxacin (68% and 28%, respectively) than DEC strains (23% and 2%, respectively) (P < 0.05). DEC-diarrhoea strains were more frequently SXT-resistant (78%) compared with DEC-control strains (65%) and commensal strains (60%) (P < 0.05). The most frequent mechanisms of antibiotic resistance in DEC strains were: for β-lactams, blaTEM (31%; 37/118); for SXT, sul2 (48%; 49/103); for tetracycline, tetA (27%; 23/84); and for chloramphenicol, cat (80%; 28/35). The genes sul1 and dfrA1, related to SXT resistance, were more frequent in the DEC-diarrhoea group (41% and 28%, respectively) than in the other two groups (P < 0.05). There was a high diversity of resistance genes in DEC, including symptomatic strains. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries International Journal of Antimicrobial Agents
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject Controlled Study|Phenotype en_US
dc.subject Infant en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject Genetic Association en_US
dc.subject Diarrhea en_US
dc.subject DNA Bacterial en_US
dc.subject Real Time Polymerase Chain Reaction|Anti-Bacterial Agents en_US
dc.subject Ampicillin en_US
dc.subject Escherichia Coli en_US
dc.subject Genetic Variability en_US
dc.subject Ciprofloxacin en_US
dc.subject Bacterium Isolation en_US
dc.subject Chloramphenicol en_US
dc.subject Cotrimoxazole en_US
dc.subject Diarrheagenic Escherichia Coli en_US
dc.subject Escherichia Coli Infections en_US
dc.subject Nalidixic Acid en_US
dc.subject Tetracycline en_US
dc.subject Antibiotic Resistance en_US
dc.subject Bacterial Strain en_US
dc.subject Antibiotics en_US
dc.subject Drug Resistance Bacterial en_US
dc.subject Children en_US
dc.subject Beta Lactam en_US
dc.subject Genes Bacterial en_US
dc.subject Antibiotic Resistance Mechanism en_US
dc.subject Bla Gene en_US
dc.subject CAT Gene en_US
dc.subject Commensal E. Coli en_US
dc.subject Commensal Escherichia Coli en_US
dc.subject Dfra1 Gene en_US
dc.subject Diarrhoeagenic E. Coli en_US
dc.subject Integrase en_US
dc.subject Sul1 Gene en_US
dc.subject Sul2 Gene en_US
dc.subject Teta Gene en_US
dc.title Molecular mechanisms of antibiotic resistance in diarrhoeagenic Escherichia coli isolated from children en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.ijantimicag.2012.07.021
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1872-7913


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