Universidad Peruana Cayetano Heredia

Anti-inflammatory activity of Mitraphylline isolated from Uncaria tomentosa bark

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dc.contributor.author Rojas-Duran, R.
dc.contributor.author González-Aspajo, G.
dc.contributor.author Ruiz-Martel, C.
dc.contributor.author Bourdy, G.
dc.contributor.author Doroteo-Ortega, V.H.
dc.contributor.author Alban-Castillo, J.
dc.contributor.author Robert, G.
dc.contributor.author Auberger, P.
dc.contributor.author Deharo, E.
dc.date.accessioned 2022-01-18T19:26:47Z
dc.date.available 2022-01-18T19:26:47Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10871
dc.description.abstract Ethnopharmacological relevance: Uncaria tomentosa (Willd. ex Roem. & Schult.) DC. (Rubiaceae) is widely used by populations living in South America to treat many ailments associated with inflammatory disorders. Mitraphylline was shown to be the major pentacyclic oxindolic alkaloid present in the bark chloroformic extract of this plant. Its activity against cytokines involved in inflammation process was tested in a murine model in vivo. Materials and methods: Mice received mitraphylline once a day for 3 days at 30 mg/kg/day by oral route. Then, they were subjected to bacterial lipopolysaccharide (LPS) endotoxin (15 mg/kg) and the LPS-induced production of 16 different cytokines was determined by Elisa multiplex. Control group received dexamethasone orally at 2 mg/kg/day. Toxicity on K565 cells and murine peritoneal macrophages, in vitro, at doses up to 100 μM was monitored by XTT-colorimetric assay. Results and conclusions: For the first time mitraphylline was tested in vivo against a large range of cytokines that play a crucial role in inflammation. Mitraphylline inhibited around 50% of the release of interleukins 1α, 1β, 17, and TNF-α. This activity was similar to dexamethasone. It also reduced almost 40% of the production of interleukin 4 (IL-4) while the corticoid did not. Lastly it did not show any toxicity on K565 cells nor murine macrophages at doses up to 100 μM. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Journal of Ethnopharmacology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Controlled Study en_US
dc.subject Animal Cell en_US
dc.subject Animal Experiment en_US
dc.subject Animal Model en_US
dc.subject Animal Tissue en_US
dc.subject In Vitro Study en_US
dc.subject In Vivo Study en_US
dc.subject Mice en_US
dc.subject Inflammation en_US
dc.subject Cytokine en_US
dc.subject Gamma Interferon en_US
dc.subject Interleukin 1beta en_US
dc.subject Cytokine Production en_US
dc.subject Cytokine Release en_US
dc.subject Treatment Duration en_US
dc.subject Granulocyte Macrophage Colony Stimulating Factor en_US
dc.subject Interleukin 10 en_US
dc.subject Interleukin 2 en_US
dc.subject Interleukin 4 en_US
dc.subject Interleukin 5 en_US
dc.subject Interleukin 6 en_US
dc.subject Tumor Necrosis Factor Alpha en_US
dc.subject Enzyme Linked Immunosorbent Assay en_US
dc.subject Bacteria (Microorganisms) en_US
dc.subject Cells Cultured en_US
dc.subject Colorimetry en_US
dc.subject Mice Inbred BALB C en_US
dc.subject Mus en_US
dc.subject Drug Isolation en_US
dc.subject Murinae en_US
dc.subject Drug Cytotoxicity en_US
dc.subject Dexamethasone en_US
dc.subject Interleukin 3 en_US
dc.subject Interleukin 12 en_US
dc.subject Interleukin 9 en_US
dc.subject Traditional Medicine en_US
dc.subject Macrophages Peritoneal en_US
dc.subject Anti-Inflammatory Agents en_US
dc.subject Monocyte Chemotactic Protein 1 en_US
dc.subject Tetrazolium en_US
dc.subject Alkaloid Derivative en_US
dc.subject Antiinflammatory Activity en_US
dc.subject Antiinflammatory Agent en_US
dc.subject Bacterium Lipopolysaccharide en_US
dc.subject Bark en_US
dc.subject Cat's Claw en_US
dc.subject Cell Survival en_US
dc.subject Chloroform en_US
dc.subject Indole Alkaloids en_US
dc.subject Interleukin 17 en_US
dc.subject Interleukin 1alpha en_US
dc.subject Lipopolysaccharides en_US
dc.subject Macrophage Inflammatory Protein 1 en_US
dc.subject Mitraphylline en_US
dc.subject Pentacyclic Oxindolic Alkaloids en_US
dc.subject Plant Bark en_US
dc.subject RANTES en_US
dc.subject Rubiaceae en_US
dc.subject TNF-Α en_US
dc.subject Uncaria Tomentosa en_US
dc.title Anti-inflammatory activity of Mitraphylline isolated from Uncaria tomentosa bark en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.jep.2012.07.015
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.05
dc.relation.issn 1872-7573


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