Universidad Peruana Cayetano Heredia

Genetic diversity, population structure and drug resistance of Mycobacterium tuberculosis in Peru

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dc.contributor.author Taype, C.A.
dc.contributor.author Agapito, J.C.
dc.contributor.author Accinelli Tanaka, Roberto Alfonso
dc.contributor.author Espinoza, J.R.
dc.contributor.author Godreuil, S.
dc.contributor.author Goodman, S.J.
dc.contributor.author Bañuls, A.L.
dc.contributor.author Shaw, M.A.
dc.date.accessioned 2022-01-18T19:26:47Z
dc.date.available 2022-01-18T19:26:47Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10881
dc.description.abstract This paper presents the first evaluation of the molecular epidemiology of . Mycobacterium tuberculosis in Peru. We characterised 323 isolates using spoligotyping and mycobacterial interspersed repetitive units variable number tandem repeats (MIRU-VNTR) typing. We aimed to determine the levels of genetic diversity and genetic differentiation among and within Peruvian isolates and the epidemiological factors which may be driving patterns of population structure and evolution of . M. tuberculosis in Peru. Our results compared to the fourth international spoligotyping database (SpolDB4) and MIRU-VNTRplus, show that the main . M. tuberculosis families present are Latin American-Mediterranean, Haarlem, T, and Beijing. Bayesian clustering recovered 15 groups in the Peruvian . M. tuberculosis isolates, among which two were composed mainly of orphans, implying the presence of native " Peruvian" strains not previously reported. Variable levels of association with drug resistance were observed, with Beijing genotypes not showing any association with multidrug resistance, while in other groups MIRU-VNTR loci 2, 23, 31, and 40 were found to be associated with the multidrug-resistant tuberculosis (MDR-TB) phenotype, suggesting that a linkage disequibrium between these MIRU and drug resistance loci may be present. Genetic differentiation was present among drug resistant and sensitive strains. Ethambutol appeared to be the main driver of differentiation, suggesting that strong selection pressure could have been exerted by drug treatment in Peru over recent years. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Infection, Genetics and Evolution
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Controlled Study en_US
dc.subject Phenotype en_US
dc.subject Genotype en_US
dc.subject Epidemiology en_US
dc.subject Drug Resistance en_US
dc.subject Genetic Variation en_US
dc.subject Selection Genetic en_US
dc.subject Population Structure en_US
dc.subject Mycobacterium Tuberculosis en_US
dc.subject Sputum en_US
dc.subject Tuberculosis en_US
dc.subject Ethambutol en_US
dc.subject Gene Locus en_US
dc.subject Bacterial Typing Techniques en_US
dc.subject Bacterium Isolate en_US
dc.subject Microbial Sensitivity Tests en_US
dc.subject Gene Linkage Disequilibrium en_US
dc.subject Genetic Variability en_US
dc.subject Linkage Disequilibrium en_US
dc.subject Molecular Epidemiology en_US
dc.subject Drug Resistance Multiple Bacterial en_US
dc.subject Bacterial Strain en_US
dc.subject Bayes Theorem en_US
dc.subject Phylogeny en_US
dc.subject Variable Number Of Tandem Repeat en_US
dc.subject Alleles en_US
dc.subject Bayesian Learning en_US
dc.subject Databases Genetic en_US
dc.subject Ethambutol en_US
dc.subject Interspersed Repeat en_US
dc.subject Minisatellite Repeats en_US
dc.subject MIRU-VNTR en_US
dc.subject Molecular Typing en_US
dc.subject Spoligotyping en_US
dc.title Genetic diversity, population structure and drug resistance of Mycobacterium tuberculosis in Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.meegid.2012.02.002
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1567-7257


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