Universidad Peruana Cayetano Heredia

TsAg5, a Taenia solium cysticercus protein with a marginal trypsin-like activity in the diagnosis of human neurocysticercosis

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dc.contributor.author Rueda, A.
dc.contributor.author Sifuentes, C.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Gutiérrez, A.H.
dc.contributor.author Piña, R.
dc.contributor.author Chile Andrade, Nancy
dc.contributor.author Carrasco, S.
dc.contributor.author Larson, S.
dc.contributor.author Mayta, H.
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Rodriguez, S.
dc.contributor.author Gutiérrez-Correa, M.
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.date.accessioned 2022-01-18T19:26:48Z
dc.date.available 2022-01-18T19:26:48Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10883
dc.description.abstract Neurocysticercosis is an endemic parasitic disease caused by Taenia solium larva. Although the mechanism of infection is not completely understood, it is likely driven by proteolytic activity that degrades the intestinal wall to facilitate oncosphere penetration and further infection. We analyzed the publicly available T. solium EST/DNA library and identified two contigs comprising a full-length cDNA fragment very similar to Echinococcus granulosus Ag5 protein. The T. solium cDNA sequence included a proteolytic trypsin-like-domain in the C-terminal region, and a thrombospondin type-1 adherence-domain in the N-terminal region. Both the trypsin-like and adherence domains were expressed independently as recombinant proteins in bacterial systems. TsAg5 showed marginal trypsin-like activity and high sequence similarity to Ag5. The purified antigens were tested in a Western immunoblot assay to diagnose human neurocysticercosis. The sensitivity of the trypsin-like-domain was 96.36% in patients infected with extraparenchymal cysts, 75.44% in patients infected with multiple cysts, and 39.62% in patients with a single cyst. Specificity was 76.70%. The thrombospondin type-1 adherence-domain was not specific for neurocysticercosis. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Molecular and Biochemical Parasitology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Protein Expression en_US
dc.subject Cyst en_US
dc.subject Proteinase en_US
dc.subject Diagnostics en_US
dc.subject Unclassified Drug en_US
dc.subject Molecular Sequence Data en_US
dc.subject Western Blotting en_US
dc.subject Echinococcus Granulosus en_US
dc.subject Taenia Solium en_US
dc.subject Swine en_US
dc.subject Bacteria (Microorganisms) en_US
dc.subject Antigens Helminth en_US
dc.subject Cysticercus en_US
dc.subject Neurocysticercosis en_US
dc.subject Recombinant Protein en_US
dc.subject Protein Degradation en_US
dc.subject Sensitivity Analysis en_US
dc.subject Taeniasis en_US
dc.subject Protein Domain en_US
dc.subject Protein Structure Tertiary en_US
dc.subject Immunoblotting en_US
dc.subject Helminth Proteins en_US
dc.subject Adherence en_US
dc.subject Amino Terminal Sequence en_US
dc.subject Antigen 5 en_US
dc.subject Complementary DNA en_US
dc.subject DNA Fragment en_US
dc.subject DNA Library en_US
dc.subject Expressed Sequence Tag en_US
dc.subject Oncosphere en_US
dc.subject Protease en_US
dc.subject Thrombospondin 1 en_US
dc.subject Trypsin en_US
dc.subject Trypsin en_US
dc.title TsAg5, a Taenia solium cysticercus protein with a marginal trypsin-like activity in the diagnosis of human neurocysticercosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.molbiopara.2011.08.003
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1872-9428


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