Universidad Peruana Cayetano Heredia

Comparison of gene expression patterns among Leishmania braziliensis clinical isolates showing a different in vitro susceptibility to pentavalent antimony

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dc.contributor.author Adaui, V.
dc.contributor.author Schnorbusch, K.
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Gutirrez, A.
dc.contributor.author Decuypere, S.
dc.contributor.author Vanaerschot, M.
dc.contributor.author De Doncker, S.
dc.contributor.author Maes, I.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Chappuis, F.
dc.contributor.author Arvalo, J.
dc.contributor.author Dujardin, J.-C.
dc.date.accessioned 2022-01-18T19:26:49Z
dc.date.available 2022-01-18T19:26:49Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10906
dc.description.abstract Introduction. Evaluation of Leishmania drug susceptibility depends on in vitro SbV susceptibility assays, which are labour-intensive and may give a biased view of the true parasite resistance. Molecular markers are urgently needed to improve and simplify the monitoring of SbV- resistance. We analysed here the gene expression profile of 21 L. braziliensis clinical isolates in vitro defined as SbV-resistant and -sensitive, in order to identify potential resistance markers. Methods. The differential expression of 13 genes involved in SbV metabolism, oxidative stress or housekeeping functions was analysed during in vitro promastigote growth. Results. Expression profiles were up-regulated for 5 genes only, each time affecting a different set of isolates (mosaic picture of gene expression). Two genes, ODC (ornithine decarboxylase) and TRYR (trypanothione reductase), showed a significantly higher expression rate in the group of SbV-resistant compared to the group of SbV-sensitive parasites (P<001). However, analysis of individual isolates showed both markers to explain only partially the drug resistance. Discussion. Our results might be explained by (i) the occurrence of a pleiotropic molecular mechanism leading to the in vitro Sb V resistance and/or (ii) the existence of different epi-phenotypes not revealed by the in vitro SbV susceptibility assays, but interfering with the gene expression patterns. en_US
dc.language.iso eng
dc.publisher Crambridge University Press
dc.relation.ispartofseries Parasitology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject article en_US
dc.subject comparative study en_US
dc.subject Animals en_US
dc.subject in vitro study en_US
dc.subject priority journal en_US
dc.subject RNA en_US
dc.subject unclassified drug en_US
dc.subject Drug Resistance en_US
dc.subject Genetic Variation en_US
dc.subject drug efficacy en_US
dc.subject quantitative analysis en_US
dc.subject Leishmania braziliensis en_US
dc.subject Leishmaniasis, Cutaneous en_US
dc.subject upregulation en_US
dc.subject real time polymerase chain reaction en_US
dc.subject sensitivity and specificity en_US
dc.subject Cell Culture Techniques en_US
dc.subject NADH, NADPH Oxidoreductases en_US
dc.subject Parasitic Sensitivity Tests en_US
dc.subject oxidative stress en_US
dc.subject parasite isolation en_US
dc.subject drug sensitivity en_US
dc.subject antimony en_US
dc.subject gene expression profiling en_US
dc.subject Gene Expression Profiling en_US
dc.subject promastigote en_US
dc.subject Antimony en_US
dc.subject trypanothione reductase en_US
dc.subject antimony resistance en_US
dc.subject housekeeping gene en_US
dc.subject clinical isolates en_US
dc.subject drug metabolism en_US
dc.subject Genetic Pleiotropy en_US
dc.subject Leishmania (Viannia) braziliensis en_US
dc.subject molecular marker en_US
dc.subject ornithine decarboxylase en_US
dc.subject Ornithine Decarboxylase en_US
dc.subject pentavalent antimony en_US
dc.subject promastigotes en_US
dc.subject RNA isolation en_US
dc.title Comparison of gene expression patterns among Leishmania braziliensis clinical isolates showing a different in vitro susceptibility to pentavalent antimony en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1017/S0031182010001095
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1469-8161


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