Universidad Peruana Cayetano Heredia

Short communication an interferon-γ ELISPOT assay with two cytotoxic T cell epitopes derived from HTLV-1 tax region 161-233 discriminates HTLV-1-associated myelopathy/tropical spastic paraparesis patients from asymptomatic HTLV-1 carriers in a peruvian population

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dc.contributor.author Best, I.
dc.contributor.author López, G.
dc.contributor.author Talledo Albujar, Michael John
dc.contributor.author MacNamara, A.
dc.contributor.author Verdonck, K.
dc.contributor.author González Lagos, Elsa Violeta
dc.contributor.author Tipismana, M.
dc.contributor.author Asquith, B.
dc.contributor.author Gotuzzo Herencia, José Eduardo
dc.contributor.author Vanham, G.
dc.contributor.author Clark, D.
dc.date.accessioned 2022-01-18T19:26:50Z
dc.date.available 2022-01-18T19:26:50Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10937
dc.description.abstract HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic and progressive disorder caused by the human T-lymphotropic virus type 1 (HTLV-1). In HTLV-1 infection, a strong cytotoxic T cell (CTL) response is mounted against the immunodominant protein Tax. Previous studies carried out by our group reported that increased IFN-γ enzyme-linked immunospot (ELISPOT) responses against the region spanning amino acids 161 to 233 of the Tax protein were associated with HAM/TSP and increased HTLV-1 proviral load (PVL). An exploratory study was conducted on 16 subjects with HAM/TSP, 13 asymptomatic carriers (AC), and 10 HTLV-1-seronegative controls (SC) to map the HAM/TSP-associated CTL epitopes within Tax region 161-233. The PVL of the infected subjects was determined and the specific CTL response was evaluated with a 6-h incubation IFN-γ ELISPOT assay using peripheral blood mononuclear cells (PBMCs) stimulated with 16 individual overlapping peptides covering the Tax region 161-233. Other proinflammatory and Th1/Th2 cytokines were also quantified in the supernatants by a flow cytometry multiplex assay. In addition, a set of human leukocyte antigen (HLA) class I alleles that bind with high affinity to the CTL epitopes of interest was determined using computational tools. Univariate analyses identified an association between ELISPOT responses to two new CTL epitopes, Tax 173-185 and Tax 181-193, and the presence of HAM/TSP as well as an increased PVL. The HLA-A*6801 allele, which is predicted to bind to the Tax 181-193 peptide, was overpresented in the HAM/TSP patients tested. en_US
dc.language.iso eng
dc.publisher Mary Ann Liebert
dc.relation.ispartofseries AIDS Research and Human Retroviruses
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject Young |Controlled Study en_US
dc.subject Carrier State en_US
dc.subject Allele en_US
dc.subject Amino Acid Sequence en_US
dc.subject Clinical |Cytokine en_US
dc.subject Cytotoxic T Lymphocyte en_US
dc.subject Enzyme Linked Immunospot Assay en_US
dc.subject Epitope Mapping en_US
dc.subject Epitopes T-Lymphocyte en_US
dc.subject Flow Cytometry en_US
dc.subject Gamma Interferon en_US
dc.subject Gene Products Tax en_US
dc.subject HLA Antigen Class 1 en_US
dc.subject HTLV 1 Associated Myelopathy en_US
dc.subject HTLV-I Infections en_US
dc.subject Human T Cell Leukemia Virus 1 en_US
dc.subject Human T-Lymphotropic Virus 1 en_US
dc.subject Interferon-Gamma en_US
dc.subject Molecular Sequence Data en_US
dc.subject Paraparesis Tropical Spastic en_US
dc.subject Peptides en_US
dc.subject Peripheral Blood Mononuclear Cell en_US
dc.subject T-Lymphocytes Cytotoxic en_US
dc.subject Tax Protein en_US
dc.subject Th1 Cell en_US
dc.subject Th2 Cell en_US
dc.subject Tropical Spastic Paraparesis en_US
dc.subject Virus Load en_US
dc.title Short communication an interferon-γ ELISPOT assay with two cytotoxic T cell epitopes derived from HTLV-1 tax region 161-233 discriminates HTLV-1-associated myelopathy/tropical spastic paraparesis patients from asymptomatic HTLV-1 carriers in a peruvian population en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1089/aid.2011.0029
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1931-8405


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