Short communication an interferon-γ ELISPOT assay with two cytotoxic T cell epitopes derived from HTLV-1 tax region 161-233 discriminates HTLV-1-associated myelopathy/tropical spastic paraparesis patients from asymptomatic HTLV-1 carriers in a peruvian population

Best, I.; Clark, D.; González Lagos, Elsa Violeta; MacNamara, A.; Gotuzzo Herencia, José Eduardo; Tipismana, M.; Asquith, B.; Verdonck, K.; Vanham, G.; Talledo Albujar, Michael John; López, G.

dc.contributor.author	Best, I.
dc.contributor.author	López, G.
dc.contributor.author	Talledo Albujar, Michael John
dc.contributor.author	MacNamara, A.
dc.contributor.author	Verdonck, K.
dc.contributor.author	González Lagos, Elsa Violeta
dc.contributor.author	Tipismana, M.
dc.contributor.author	Asquith, B.
dc.contributor.author	Gotuzzo Herencia, José Eduardo
dc.contributor.author	Vanham, G.
dc.contributor.author	Clark, D.
dc.date.accessioned	2022-01-18T19:26:50Z
dc.date.available	2022-01-18T19:26:50Z
dc.date.issued	2011
dc.identifier.uri	https://hdl.handle.net/20.500.12866/10937
dc.description.abstract	HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic and progressive disorder caused by the human T-lymphotropic virus type 1 (HTLV-1). In HTLV-1 infection, a strong cytotoxic T cell (CTL) response is mounted against the immunodominant protein Tax. Previous studies carried out by our group reported that increased IFN-γ enzyme-linked immunospot (ELISPOT) responses against the region spanning amino acids 161 to 233 of the Tax protein were associated with HAM/TSP and increased HTLV-1 proviral load (PVL). An exploratory study was conducted on 16 subjects with HAM/TSP, 13 asymptomatic carriers (AC), and 10 HTLV-1-seronegative controls (SC) to map the HAM/TSP-associated CTL epitopes within Tax region 161-233. The PVL of the infected subjects was determined and the specific CTL response was evaluated with a 6-h incubation IFN-γ ELISPOT assay using peripheral blood mononuclear cells (PBMCs) stimulated with 16 individual overlapping peptides covering the Tax region 161-233. Other proinflammatory and Th1/Th2 cytokines were also quantified in the supernatants by a flow cytometry multiplex assay. In addition, a set of human leukocyte antigen (HLA) class I alleles that bind with high affinity to the CTL epitopes of interest was determined using computational tools. Univariate analyses identified an association between ELISPOT responses to two new CTL epitopes, Tax 173-185 and Tax 181-193, and the presence of HAM/TSP as well as an increased PVL. The HLA-A*6801 allele, which is predicted to bind to the Tax 181-193 peptide, was overpresented in the HAM/TSP patients tested.	en_US
dc.language.iso	eng
dc.publisher	Mary Ann Liebert
dc.relation.ispartofseries	AIDS Research and Human Retroviruses
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Humans	en_US
dc.subject	Peru	en_US
dc.subject	Young \|Controlled Study	en_US
dc.subject	Carrier State	en_US
dc.subject	Allele	en_US
dc.subject	Amino Acid Sequence	en_US
dc.subject	Clinical \|Cytokine	en_US
dc.subject	Cytotoxic T Lymphocyte	en_US
dc.subject	Enzyme Linked Immunospot Assay	en_US
dc.subject	Epitope Mapping	en_US
dc.subject	Epitopes T-Lymphocyte	en_US
dc.subject	Flow Cytometry	en_US
dc.subject	Gamma Interferon	en_US
dc.subject	Gene Products Tax	en_US
dc.subject	HLA Antigen Class 1	en_US
dc.subject	HTLV 1 Associated Myelopathy	en_US
dc.subject	HTLV-I Infections	en_US
dc.subject	Human T Cell Leukemia Virus 1	en_US
dc.subject	Human T-Lymphotropic Virus 1	en_US
dc.subject	Interferon-Gamma	en_US
dc.subject	Molecular Sequence Data	en_US
dc.subject	Paraparesis Tropical Spastic	en_US
dc.subject	Peptides	en_US
dc.subject	Peripheral Blood Mononuclear Cell	en_US
dc.subject	T-Lymphocytes Cytotoxic	en_US
dc.subject	Tax Protein	en_US
dc.subject	Th1 Cell	en_US
dc.subject	Th2 Cell	en_US
dc.subject	Tropical Spastic Paraparesis	en_US
dc.subject	Virus Load	en_US
dc.title	Short communication an interferon-γ ELISPOT assay with two cytotoxic T cell epitopes derived from HTLV-1 tax region 161-233 discriminates HTLV-1-associated myelopathy/tropical spastic paraparesis patients from asymptomatic HTLV-1 carriers in a peruvian population	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1089/aid.2011.0029
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn	1931-8405