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A new approach for pyrazinamide susceptibility testing in mycobacterium tuberculosis

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dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Loli, S.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Gutierrez, Andrés
dc.contributor.author Fuentes, P.
dc.contributor.author Cotrina, M.
dc.contributor.author Kirwan, D.
dc.contributor.author Sheen Cortavarria, Patricia
dc.date.accessioned 2022-01-18T19:26:51Z
dc.date.available 2022-01-18T19:26:51Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10942
dc.description.abstract Background: Pyrazinamide (PZA) is an important drug in the treatment of tuberculosis. Microbiological methods of PZA susceptibility testing are controversial and have low reproducibility. After conversion of PZA into pyrazinoic acid (POA) by the bacterial pyrazinamidase enzyme, the drug is expelled from the bacteria by an efflux pump. Objective: To evaluate the rate of POA extrusion from Mycobacterium tuberculosis as a parameter to detect PZA resistance. Methods: The rate of POA extrusion and PZA susceptibility determined by BACTEC 460 were measured for 34 strains in a previous study. PZA resistance was modeled in a logistic regression with the pyrazinoic efflux rate. Result: POA efflux rate predicted PZA resistance with 70.83%-92.85% sensitivity and 100% specificity compared with BACTEC 460. Conclusion: POA efflux rate could be a useful tool for predicting PZA resistance in M. tuberculosis. Further exploration of this approach may lead to the development of new tools for diagnosing PZA resistance, which may be of public health importance. en_US
dc.language.iso eng
dc.publisher Mary Ann Liebert
dc.relation.ispartofseries Microbial Drug Resistance
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Reproducibility Of Results en_US
dc.subject Carrier Protein en_US
dc.subject Mycobacterium Tuberculosis en_US
dc.subject Sensitivity And Specificity en_US
dc.subject Culture Medium en_US
dc.subject Kinetics en_US
dc.subject Bacterium Culture en_US
dc.subject Tuberculosis Pulmonary en_US
dc.subject Antitubercular Agents en_US
dc.subject Pyrazinamide en_US
dc.subject Bacteria (Microorganisms) en_US
dc.subject Bacterial Enzyme en_US
dc.subject Intermethod Comparison en_US
dc.subject Microbial Sensitivity Tests en_US
dc.subject Antibiotic Sensitivity en_US
dc.subject Antibiotic Resistance en_US
dc.subject Regression Analysis en_US
dc.subject Drug Resistance Bacterial en_US
dc.subject Prediction en_US
dc.subject Pyrazinamide en_US
dc.subject Biological Transport en_US
dc.subject Biotransformation en_US
dc.subject Parameters en_US
dc.subject Pyrazinoic Acid en_US
dc.title A new approach for pyrazinamide susceptibility testing in mycobacterium tuberculosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1089/mdr.2011.0207
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.05
dc.relation.issn 1931-8448


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