Universidad Peruana Cayetano Heredia

Quantitative real-time polymerase Chain reaction for enteropathogenic escherichia coli: A tool for investigation of asymptomatic versus symptomatic infections

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dc.contributor.author Barletta, F.
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Mercado, E.
dc.contributor.author Ruiz, Joaquim
dc.contributor.author Ecker, L.
dc.contributor.author Lopez, G.
dc.contributor.author Mispireta, M.
dc.contributor.author Gil, A.I.
dc.contributor.author Lanata, C.F.
dc.contributor.author Cleary, T.G.
dc.date.accessioned 2022-01-18T19:26:51Z
dc.date.available 2022-01-18T19:26:51Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10945
dc.description.abstract Background. Enteropathogenic Escherichia coli (EPEC) strains are pediatric pathogens commonly isolated from both healthy and sick children with diarrhea in areas of endemicity. The aim of this study was to compare the bacterial load of EPEC isolated from stool samples from children with and without diarrhea to determine whether bacterial load might be a useful tool for further study of this phenomenon. Methods. EPEC was detected by polymerase chain reaction (PCR) of colonies isolated on MacConkey plates from 53 diarrheal and 90 healthy children aged <2 years. DNA was isolated from stool samples by cetyltrimethylammonium bromide extraction. To standardize quantification by quantitative real-time PCR (qRT-PCR), the correlation between fluorescence threshold cycle and copy number of the intimin gene of EPEC E2348/69 was determined. Results. The detection limit of qRT-PCR was 5 bacteria/mg stool. The geometric mean load in diarrhea was 299 bacteria/mg (95% confidence interval [CI], 77-1164 bacteria/mg), compared with 29 bacteria/mg (95% CI, 10-87 bacteria/mg) in control subjects (P =. 016). Bacterial load was significantly higher in children with diarrhea than in control subjects among children <12 months of age (178 vs 5 bacteria/mg; P =. 006) and among children with EPEC as the sole pathogen (463 vs 24 bacteria/mg; P =. 006). Conclusions. EPEC load measured by qRT-PCR is higher in diarrheal than in healthy children. qRT-PCR may be useful to study the relationship between disease and colonization in settings of endemicity. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartofseries Clinical Infectious Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Cohort Studies en_US
dc.subject Controlled Study en_US
dc.subject Major Clinical Study en_US
dc.subject Nucleotide Sequence en_US
dc.subject Asymptomatic Infection en_US
dc.subject Infant en_US
dc.subject Diarrhea en_US
dc.subject Newborn en_US
dc.subject Feces en_US
dc.subject Bacterial Load en_US
dc.subject Real Time Polymerase Chain Reaction en_US
dc.subject Feces Analysis en_US
dc.subject Bacterium Isolation en_US
dc.subject Escherichia Coli Infections en_US
dc.subject Asymptomatic Diseases en_US
dc.subject Bacterial Load en_US
dc.subject Cetrimide en_US
dc.subject Copy Number Variation en_US
dc.subject DNA Isolation en_US
dc.subject Enteropathogenic Escherichia Coli en_US
dc.subject Gene Dosage en_US
dc.subject Intimin en_US
dc.title Quantitative real-time polymerase Chain reaction for enteropathogenic escherichia coli: A tool for investigation of asymptomatic versus symptomatic infections en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1093/cid/cir730
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1537-6591


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