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Chemical inhibition of potato ABA-8'-hydroxylase activity alters in vitro and in vivo ABA metabolism and endogenous ABA levels but does not affect potato microtuber dormancy duration

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dc.contributor.author Suttle, J.C.
dc.contributor.author Abrams, S.R.
dc.contributor.author De Stefano-Beltrán, L.
dc.contributor.author Huckle, L.L.
dc.date.accessioned 2022-01-18T19:26:51Z
dc.date.available 2022-01-18T19:26:51Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10952
dc.description.abstract The effects of azole-type P450 inhibitors and two metabolism-resistant abscisic acid (ABA) analogues on in vitro ABA-8'-hydroxylase activity, in planta ABA metabolism, endogenous ABA content, and tuber meristem dormancy duration were examined in potato (Solanum tuberosum L. cv. Russet Burbank). When functionally expressed in yeast, three potato CYP707A genes were demonstrated to encode enzymatically active ABA-8'-hydroxylases with micromolar affinities for (+)-ABA. The in vitro activity of the three enzymes was inhibited by the P450 azole-type inhibitors ancymidol, paclobutrazol, diniconazole, and tetcyclasis, and by the 8'-acetylene-and 8'-methylene-ABA analogues, with diniconazole and tetcyclasis being the most potent inhibitors. The in planta metabolism of [ 3H](±)-ABA to phaseic acid and dihydrophaseic acid in tuber meristems was inhibited by diniconazole, tetcyclasis, and to a lesser extent by 8'-acetylene-and 8'-methylene-ABA. Continuous exposure of in vitro generated microtubers to diniconazole resulted in a 2-fold increase in endogenous ABA content and a decline in dihydrophaseic acid content after 9 weeks of development. Similar treatment with 8'-acetylene-ABA had no effects on the endogenous contents of ABA or phaseic acid but reduced the content of dihydrophaseic acid. Tuber meristem dormancy progression was determined ex vitro in control, diniconazole-, and 8'-acetylene-ABA-treated microtubers following harvest. Continuous exposure to diniconazole during microtuber development had no effects on subsequent sprouting at any time point. Continuous exposure to 8'-acetylene-ABA significantly increased the rate of microtuber sprouting. The results indicate that, although a decrease in ABA content is a hallmark of tuber dormancy progression, the decline in ABA levels is not a prerequisite for dormancy exit and the onset of tuber sprouting. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartofseries Journal of Experimental Botany
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject article en_US
dc.subject genetics en_US
dc.subject metabolism en_US
dc.subject physiology en_US
dc.subject gene expression regulation en_US
dc.subject drug effect en_US
dc.subject enzymology en_US
dc.subject triazole derivative en_US
dc.subject Triazoles en_US
dc.subject drug derivative en_US
dc.subject chemistry en_US
dc.subject Pyrimidines en_US
dc.subject pyrimidine derivative en_US
dc.subject drug antagonism en_US
dc.subject potato en_US
dc.subject Solanum tuberosum en_US
dc.subject ABA en_US
dc.subject ABA-8'-hydroxylase en_US
dc.subject abscisic acid en_US
dc.subject Abscisic Acid en_US
dc.subject abscisic acid 8' hydroxylase en_US
dc.subject abscisic acid 8'-hydroxylase en_US
dc.subject ancymidol en_US
dc.subject Cytochrome P-450 Enzyme System en_US
dc.subject cytochrome P450 en_US
dc.subject diniconazole en_US
dc.subject dormancy en_US
dc.subject fused heterocyclic rings en_US
dc.subject Gene Expression Regulation, Plant en_US
dc.subject Heterocyclic Compounds with 4 or More Rings en_US
dc.subject meristem en_US
dc.subject Meristem en_US
dc.subject microtuber en_US
dc.subject mixed-function oxidase en_US
dc.subject P450 en_US
dc.subject paclobutrazol en_US
dc.subject phytohormone en_US
dc.subject Plant Growth Regulators en_US
dc.subject Plant Proteins en_US
dc.subject plant tuber en_US
dc.subject Plant Tubers en_US
dc.subject Solanum tuberosum L. en_US
dc.subject tetcyclasis en_US
dc.subject vegetable protein en_US
dc.title Chemical inhibition of potato ABA-8'-hydroxylase activity alters in vitro and in vivo ABA metabolism and endogenous ABA levels but does not affect potato microtuber dormancy duration en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1093/jxb/ers146
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.10
dc.relation.issn 1460-2431


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