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Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children

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dc.contributor.author Contreras, C.A.
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Ruiz, J.
dc.contributor.author Lacher, D.W.
dc.contributor.author Durand Vara, David Percy
dc.contributor.author Debroy, C.
dc.contributor.author Lanata, C.F.
dc.contributor.author Cleary, T.G.
dc.date.accessioned 2022-01-18T19:26:52Z
dc.date.available 2022-01-18T19:26:52Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10968
dc.description.abstract The aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded esp and tir genes among 181 enteropathogenic Escherichia coli (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed espA, espB, espD and tir alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 %) than from diarrhoeal samples (90/936, 9.6 %) (P<0.001); 28.9% of diarrhoeal and 17.6% of control samples were typical EPEC (tEPEC). The distribution of espA alleles (alpha, beta, beta2 and gamma) and espD alleles (alpha, beta, gamma and a new variant, espD-N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (P<0.05). espD-alpha was more common among acute episodes (P<0.05). espB typing resulted in five alleles (alpha, beta, gamma and two new suballeles, espB-alpha2 and espB-alpha3), while tir-beta and tir-gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of espA, espB, espD and tir alleles were found; the most prevalent combination was espA-beta, espB-beta, espD-beta, tir-beta (34/181 strains), which was more frequent among tEPEC strains (P,0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster. en_US
dc.language.iso eng
dc.publisher Microbiology Society
dc.relation.ispartofseries Journal of Medical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject Controlled Study en_US
dc.subject Genotype en_US
dc.subject Nucleotide Sequence en_US
dc.subject Infant en_US
dc.subject Genetic Variation en_US
dc.subject Diarrhea en_US
dc.subject Molecular Sequence Data en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject Sequence Analysis DNA en_US
dc.subject Gene Frequency en_US
dc.subject Genetic Association en_US
dc.subject DNA Bacterial en_US
dc.subject Molecular Epidemiology en_US
dc.subject Polymorphism Restriction Fragment Length en_US
dc.subject Bacterial Gene en_US
dc.subject Gene Locus en_US
dc.subject Genetic Variability en_US
dc.subject Bacterium Isolation en_US
dc.subject Escherichia Coli Infections en_US
dc.subject Phosphoproteins en_US
dc.subject Enteropathogenic Escherichia Coli en_US
dc.subject Escherichia Coli Proteins en_US
dc.subject DNA Fingerprinting en_US
dc.subject Espa Alpha Gene en_US
dc.subject Espa Beta Gene en_US
dc.subject Espa Beta2 Gene en_US
dc.subject Espa Gamma Gene en_US
dc.subject Espb Alpha Gene en_US
dc.subject Espb Alpha2 Gene en_US
dc.subject Espb Alpha3 Gene en_US
dc.subject Espb Beta Gene en_US
dc.subject Espb Gamma Gene en_US
dc.subject Espd Alpha Gene en_US
dc.subject Espd Beta Gene en_US
dc.subject Espd Gamma Gene en_US
dc.subject Espd N1 Gene en_US
dc.subject Microbial Diversity en_US
dc.subject Tir Alpha Gene en_US
dc.subject Tir Alpha2 Gene en_US
dc.subject Tir Beta Gene en_US
dc.subject Tir Gamma Gene en_US
dc.subject Tir Gamma2 Gene en_US
dc.title Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1099/jmm.0.045443-0
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#
dc.relation.issn 1473-5644


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