Universidad Peruana Cayetano Heredia

Development of an automated MODS plate reader to detect early growth of Mycobacterium tuberculosis

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dc.contributor.author Comina, G.
dc.contributor.author Mendoza, D.
dc.contributor.author Velazco, A.
dc.contributor.author Coronel, J.
dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Moore, David Alexander James
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.date.accessioned 2022-01-18T19:26:52Z
dc.date.available 2022-01-18T19:26:52Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10971
dc.description.abstract In this work, an automated microscopic observation drug susceptibility (MODS) plate reader has been developed. The reader automatically handles MODS plates and after autofocussing digital images are acquired of the characteristic microscopic cording structures ofMycobacterium tuberculosis, which are the identification method utilized in the MODS technique to detect tuberculosis and multidrug resistant tuberculosis. In conventional MODS, trained technicians manually move the MODS plate on the stage of an inverted microscope while trying to locate and focus upon the characteristic microscopic cording colonies. In centres with high tuberculosis diagnostic demand, sufficient time may not be available to adequately examine all cultures. An automated reader would reduce labour time and the handling ofM. tuberculosiscultures by laboratory personnel. Two hundred MODS culture images (100 from tuberculosis positive and 100 from tuberculosis negative sputum samples confirmed by a standard MODS reading using a commercial microscope) were acquired randomly using the automated MODS plate reader. A specialist analysed these digital images with the help of a personal computer and designated them asM. tuberculosispresent or absent. The specialist considered four images insufficiently clear to permit a definitive reading. The readings from the 196 valid images resulted in a 100% agreement with the conventional nonautomated standard reading. The automated MODS plate reader combined with open-source MODS pattern recognition software provides a novel platform for high throughput automated tuberculosis diagnosis. en_US
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartofseries Journal of Microscopy
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject article en_US
dc.subject non|priority journal en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject tuberculosis en_US
dc.subject bacterium culture en_US
dc.subject Antitubercular Agents en_US
dc.subject multidrug resistant tuberculosis en_US
dc.subject algorithm en_US
dc.subject laboratory personnel en_US
dc.subject Microbial Sensitivity Tests en_US
dc.subject bacterial growth en_US
dc.subject microscopy en_US
dc.subject Colony Count, Microbial en_US
dc.subject Software en_US
dc.subject MODS en_US
dc.subject image analysis en_US
dc.subject Autofocussing algorithm en_US
dc.subject Automated MODS en_US
dc.subject Automation en_US
dc.subject electronics en_US
dc.subject microscopic observation drug susceptibility plate reader en_US
dc.subject MODS plate reader en_US
dc.title Development of an automated MODS plate reader to detect early growth of Mycobacterium tuberculosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/j.1365-2818.2010.03477.x
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#
dc.relation.issn 1365-2818


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