Universidad Peruana Cayetano Heredia

A novel method for the rapid and prospective identification of Beijing Mycobacterium tuberculosis strains by high-resolution melting analysis

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dc.contributor.author Alonso, M.
dc.contributor.author Navarro, Y.
dc.contributor.author Barletta, F.
dc.contributor.author Martínez Lirola, M.
dc.contributor.author Gotuzzo Herencia, José Eduardo
dc.contributor.author Bouza, E.
dc.contributor.author de Viedma, D.G.
dc.date.accessioned 2022-01-18T19:26:53Z
dc.date.available 2022-01-18T19:26:53Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10983
dc.description.abstract Genotypic analysis of Mycobacterium tuberculosis (MTB) has enabled the definition of several lineages. The Beijing family, which is considered highly virulent and transmissible, has been associated with resistance in certain settings and involved in severe outbreaks, making it one of the most closely-monitored lineages. Therefore, rapid prospective identification of Beijing MTB strains could be relevant. In the present study, we evaluate a real-time PCR followed by high-resolution melting (HRM) based on the identification of a single nucleotide polymorphism (SNP) in the Rv2629 gene which defines Beijing lineage (A191C for Beijing genotype and A191A for non-Beijing genotype). This combined methodology efficiently differentiated Beijing and non-Beijing strains in 100% of the isolates from a collection of reference strains without requiring specific DNA probes. Additionally, HRM was able to assign a Beijing/non-Beijing genotype in 90.9% of the respiratory specimens assayed. Its applicability was tested on a Peruvian sample of circulating MTB strains, in which it identified 10.7% as belonging to the Beijing genotype; this proportion reached 20% in the North Lima area. HRM analysis of the A191C SNP is a rapid, reliable, and sensitive method for the efficient prospective survey of high-risk Beijing MTB strains, even in developing settings where MTB culture is often not available. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Clinical Microbiology and Infection
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Genotype en_US
dc.subject Single Nucleotide Polymorphism en_US
dc.subject Gene en_US
dc.subject Mycobacterium Tuberculosis en_US
dc.subject Real Time Polymerase Chain Reaction en_US
dc.subject Tuberculosis en_US
dc.subject Bacterium Identification en_US
dc.subject Bacterial Strain en_US
dc.subject Analytic Method en_US
dc.subject Reliability en_US
dc.subject Sensitivity Analysis en_US
dc.subject Beijing Mycobacterium Tuberculosis en_US
dc.subject Cell Lineage en_US
dc.subject DNA Probe en_US
dc.subject Genetic Linkage en_US
dc.subject High-Resolution Melting Analysis en_US
dc.title A novel method for the rapid and prospective identification of Beijing Mycobacterium tuberculosis strains by high-resolution melting analysis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/j.1469-0691.2010.03234.x
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1469-0691


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