Universidad Peruana Cayetano Heredia

Sensitive in vitro system to assess morphological and biochemical effects of praziquantel and albendazole on Taenia solium cysts

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dc.contributor.author Mahanty, S.
dc.contributor.author Paredes, A.
dc.contributor.author Marzal, M.
dc.contributor.author Gonzalez, E.
dc.contributor.author Rodriguez, S.
dc.contributor.author Dorny, P.
dc.contributor.author Guerra-Giraldez, C.
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Nash, T.
dc.date.accessioned 2022-01-18T19:26:53Z
dc.date.available 2022-01-18T19:26:53Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10991
dc.description.abstract Neurocysticercosis resulting from Taenia solium infections is a major cause of adult-acquired seizures worldwide. Disease is caused by larval cysts, and treatment consists of the anthelmintic drugs albendazole or praziquantel. There are no standard methods to assess drug activity to T. solium cysts in vitro. Morphological, functional, and biochemical changes that might reflect damaging (inhibiting, cytotoxic) drug effects were analyzed after exposure of cysts to albendazole sulfoxide (ABZ-SO), the major active metabolite of the drug in vivo, praziquantel (PZQ), or combinations of both. PZQ exposure led to a decrease in cyst size and inhibition of evagination, whereas ABZ-SO exposure resulted in minimal changes. Alkaline phosphatase (AP) is normally secreted by cysts, and both drugs inhibited AP secretion at concentrations of 5 and 50 ng/ml for PZQ and ABZ-SO, respectively. Some combinations of both drugs resulted in additive and/or synergistic activities. Parasite-specific antigen, detected in the cerebrospinal fluid and blood of infected patients, is also normally secreted by T. solium cysts. Antigen secretion was similarly inhibited by ABZ-SO and PZQ and a combination of both drugs, suggesting that inhibition of secretion is a common downstream consequence of the activities of both drugs. These studies establish quantitative methods to measure in vitro anthelmintic activity and suggest combination therapy with ABZ-SO and PZQ may have clinical benefit. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Antimicrobial Agents and Chemotherapy
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animal Experiment en_US
dc.subject In Vitro Study|Morphology en_US
dc.subject Parasite Antigen en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject Drug Effect en_US
dc.subject Albendazole en_US
dc.subject Cyst (Resting Stage) en_US
dc.subject Taenia Solium en_US
dc.subject Anthelmintics en_US
dc.subject Praziquantel en_US
dc.subject Albendazole Sulfoxide en_US
dc.subject Alkaline Phosphatase en_US
dc.subject Praziquantel en_US
dc.subject Drug Potentiation en_US
dc.title Sensitive in vitro system to assess morphological and biochemical effects of praziquantel and albendazole on Taenia solium cysts en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/AAC.00761-10
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.21
dc.relation.issn 1098-6596


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