Universidad Peruana Cayetano Heredia

Serological diagnosis of lung cystic hydatid disease using the synthetic p176 peptide

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dc.contributor.author Santivañez, S.J.
dc.contributor.author Arias, P.
dc.contributor.author Portocarrero, M.
dc.contributor.author Rodriguez, S.
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Gavidia, C.M.
dc.contributor.author García Lescano, Héctor Hugo
dc.date.accessioned 2022-01-18T19:26:53Z
dc.date.available 2022-01-18T19:26:53Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10995
dc.description.abstract Cystic hydatid disease (CHD) is a worldwide zoonosis caused by the larval stage of the dog tapeworm Echinococcus granulosus. Diagnosis is based on imagenological tools (abdominal ultrasound, chest X-rays, or computed tomography [CT] scan). Serological antibody-detecting assays, using diverse native antigens, have been used as a supportive diagnostic tool, but their sensitivities and specificities differ greatly. The use of synthetic peptides as antigens should provide more reliability and allow better assessment and comparison of test formats and case series. The synthetic peptide p176, corresponding to the N-terminal extreme of the subunit of antigen B (AgB8/1), has shown promising performances for diagnosis of CHD. We evaluated the performance of the synthetic peptide p176 for the diagnosis of pulmonary hydatid disease in an enzyme-linked immunosorbent assay (ELISA) format. Sixty-one serum samples from patients with a diagnosis of pulmonary hydatidosis confirmed by surgery and 128 from healthy volunteers were tested. The overall sensitivity and specificity of the p176 ELISA for lung CHD were 78.69% and 96.88%, respectively. On bivariate analysis, positive serum antibody reactions were associated with the presence of complications and with the number of cysts (single/multiple). Only the presence of persistent complications significantly associated with seropositivity on multivariate logistic regression analysis (odds ratio [OR], 9.58; 95% confidence interval [CI], 2.15 to 42.6; P = 0.003). The p176 ELISA performs well for the diagnosis of lung CHD and adds an easily reproducible diagnostic assay to the existing diagnostic tools. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Clinical and Vaccine Immunology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Animals en_US
dc.subject unclassified drug en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject Sensitivity and Specificity en_US
dc.subject diagnostic value en_US
dc.subject Serologic Tests en_US
dc.subject Echinococcus granulosus en_US
dc.subject enzyme linked immunosorbent assay en_US
dc.subject diagnostic accuracy en_US
dc.subject antibody blood level en_US
dc.subject Echinococcosis, Pulmonary en_US
dc.subject infection complication en_US
dc.subject Lipoproteins en_US
dc.subject lung hydatid cyst en_US
dc.subject lung surgery en_US
dc.subject parasite examination en_US
dc.subject peptide analysis en_US
dc.subject synthetic p176 peptide en_US
dc.subject synthetic peptide en_US
dc.title Serological diagnosis of lung cystic hydatid disease using the synthetic p176 peptide en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/CVI.05540-11
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.03
dc.relation.issn 1556-679X


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