Universidad Peruana Cayetano Heredia

Fecal leukocytes in children infected with diarrheagenic Escherichia coli.

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dc.contributor.author Mercado, Erik H.
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Ecker, Lucie
dc.contributor.author Cabello Vílchez, Alfonso Martín
dc.contributor.author Durand Vara, David Percy
dc.contributor.author Barletta, Francesca
dc.contributor.author Molina, Margarita
dc.contributor.author Gil, Ana I.
dc.contributor.author Huicho Oriundo, Luis
dc.contributor.author Lanata, Claudio F.
dc.contributor.author Cleary, Thomas G.
dc.date.accessioned 2022-01-18T19:26:53Z
dc.date.available 2022-01-18T19:26:53Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10997
dc.description.abstract The purpose of this study was to determine the presence and quantity of fecal leukocytes in children infected with diarrheagenic Escherichia coli and to compare these levels between diarrhea and control cases. We analyzed 1,474 stool samples from 935 diarrhea episodes and 539 from healthy controls of a cohort study of children younger than 2 years of age in Lima, Peru. Stools were analyzed for common enteric pathogens, and diarrheagenic E. coli isolates were studied by a multiplex real-time PCR. Stool smears were stained with methylene blue and read by a blinded observer to determine the number of polymorphonuclear leukocytes per high-power field (L/hpf). Fecal leukocytes at >10 L/hpf were present in 11.8% (110/935) of all diarrheal episodes versus 1.1% (6/539) in controls (P < 0.001). Among stool samples with diarrheagenic E. coli as the only pathogen isolated (excluding coinfection), fecal leukocytes at >10 L/hpf were present in 8.5% (18/212) of diarrhea versus 1.3% (2/157) of control samples (P < 0.01). Ninety-five percent of 99 diarrheagenic E. coli diarrhea samples were positive for fecal lactoferrin. Adjusting for the presence of blood in stools, age, sex, undernutrition, and breastfeeding, enterotoxigenic E. coli (ETEC) isolation as a single pathogen, excluding coinfections, was highly associated with the presence of fecal leukocytes (>10 L/hpf) with an odds ratio (OR) of 4.1 (95% confidence interval [CI], 1.08 to 15.51; P < 0.05). Although diarrheagenic E. coli was isolated with similar frequencies in diarrhea and control samples, clearly it was associated with a more inflammatory response during symptomatic infection; however, in general, these pathogens elicited a mild inflammatory response. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Peru en_US
dc.subject controlled study en_US
dc.subject age distribution en_US
dc.subject Infant en_US
dc.subject sex difference en_US
dc.subject quantitative analysis en_US
dc.subject human cell en_US
dc.subject Diarrhea en_US
dc.subject Feces en_US
dc.subject breast feeding en_US
dc.subject malnutrition en_US
dc.subject mixed infection en_US
dc.subject Escherichia coli en_US
dc.subject bacterium isolate en_US
dc.subject diarrheagenic Escherichia coli en_US
dc.subject enterotoxigenic Escherichia coli en_US
dc.subject Escherichia coli infection en_US
dc.subject leukocyte count en_US
dc.subject lactoferrin en_US
dc.subject enteropathogenic Escherichia coli en_US
dc.subject feces level en_US
dc.subject neutrophil count en_US
dc.subject Neutrophils en_US
dc.subject occult blood en_US
dc.title Fecal leukocytes in children infected with diarrheagenic Escherichia coli. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.02199-10
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1098-660X


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