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Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis

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dc.contributor.author Boggild, A.K.
dc.contributor.author Ramos, A.P.
dc.contributor.author Valencia, B.M.
dc.contributor.author Veland, N.
dc.contributor.author Calderon, F.
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.contributor.author Low, D.E.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.date.accessioned 2022-01-18T19:26:53Z
dc.date.available 2022-01-18T19:26:53Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10998
dc.description.abstract We compared traditional cutaneous leishmaniasis diagnostic methods to filter paper lesion impression (FPLI) PCR for secondarily infected ulcers and nonulcerative lesions. The sensitivity and specificity of FPLI PCR for secondarily infected lesions (n = 8) were 100%. In primarily nonulcerative lesions (n = 15), the sensitivity of FPLI PCR was inferior to that of pooled-invasive-specimen PCR (72.7% versus 100%) (P = 0.10). FPLI PCR is sensitive, specific, and unlike invasive procedures, can be used in secondarily infected ulcers. Invasive specimen collection is superior in nonulcerative lesions. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject controlled study en_US
dc.subject major clinical study en_US
dc.subject priority journal en_US
dc.subject polymerase chain reaction en_US
dc.subject DNA, Protozoan en_US
dc.subject Leishmania braziliensis en_US
dc.subject Leishmaniasis, Cutaneous en_US
dc.subject leishmanin skin test en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject skin leishmaniasis en_US
dc.subject Sensitivity and Specificity en_US
dc.subject diagnostic test accuracy study en_US
dc.subject diagnostic value en_US
dc.subject microbiological examination en_US
dc.subject restriction fragment length polymorphism en_US
dc.subject skin ulcer en_US
dc.subject Leishmania en_US
dc.subject Leishmania lainsoni en_US
dc.subject Skin Ulcer en_US
dc.subject intermethod comparison en_US
dc.subject leishmania peruviana en_US
dc.subject predictive value en_US
dc.subject smear en_US
dc.subject Specimen Handling en_US
dc.subject Parasitology en_US
dc.subject species identification en_US
dc.subject filter paper lesion impression polymerase chain reaction en_US
dc.subject Geimsa stained smear en_US
dc.subject non invasive measurement en_US
dc.subject protozoon culture en_US
dc.subject secondary infection en_US
dc.title Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.02457-10
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1098-660X


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