Universidad Peruana Cayetano Heredia

Serial kinetics of the antibody response against the complete Brucella melitensis ORFeome in focal vertebral brucellosis

Mostrar el registro sencillo del ítem

dc.contributor.author Cannella, A.P.
dc.contributor.author Lin, J.C.
dc.contributor.author Liang, L.
dc.contributor.author Atluri, V.
dc.contributor.author Gotuzzo Herencia, José Eduardo
dc.contributor.author Felgner, P.L.
dc.contributor.author Tsolis, R.M.
dc.contributor.author Vinetz, Joseph Michael
dc.date.accessioned 2022-01-18T19:26:53Z
dc.date.available 2022-01-18T19:26:53Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/11000
dc.description.abstract Human brucellosis is a common zoonosis worldwide. Here we present a case of focal vertebral brucellosis in a 71-year-old Mexican-American woman who contracted infection from unpasteurized goat milk. Standard agglutination serology was negative; the diagnosis was established by the isolation of Brucella melitensis from abscess fluid. A B. melitensis protein microarray comprised of nearly all proteins encoded by the bacterial genome was used to determine the kinetics of this patient's antibody responses to the complete collection of open reading frames existing in the genome (ORFeome). Three patterns of antibody responses against B. melitensis antigens were seen for serum samples obtained on days 0 (pretreatment), 14, 49, 100, and 180: (i) stable titers over time, (ii) a steady fall in titers, and (iii) an initial rise in titers followed by declining titers. Sera from this patient with chronic brucellosis recognized some of the same B. melitensis proteins as those recognized by sera from acute/subacute, blood culture-positive brucellosis patients but also recognized a distinct set of proteins. This study is the first to determine the kinetics of the human antibody responses to the complete repertoire of proteins encoded by a bacterial genome and demonstrates fundamentally different immunopathogenetic mechanisms between acute human brucellosis and chronic human brucellosis. While an extension of these findings to a larger patient population is necessary, these findings have important clinical and diagnostic implications and lead toward new insights into the fundamental immunopathogenesis of brucellosis. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Nuclear Magnetic Resonance Imaging en_US
dc.subject Case Report en_US
dc.subject Fever en_US
dc.subject Rifampicin en_US
dc.subject Time Factors en_US
dc.subject Doxycycline en_US
dc.subject Milk en_US
dc.subject Antibody Response en_US
dc.subject Antibody Titer en_US
dc.subject Blood Culture en_US
dc.subject Brucella Melitensis en_US
dc.subject Brucellosis en_US
dc.subject Goats en_US
dc.subject Sweating en_US
dc.subject Magnetic Resonance Imaging en_US
dc.subject Bacterium Isolation en_US
dc.subject Gentamicin en_US
dc.subject Osteomyelitis en_US
dc.subject Psoas Abscess en_US
dc.subject Antibodies Bacterial en_US
dc.subject Antigens Bacterial en_US
dc.subject Bone Infection en_US
dc.subject Serum en_US
dc.subject Pain en_US
dc.subject Antigen Recognition en_US
dc.subject Bacterial Genome en_US
dc.subject Bone Biopsy en_US
dc.subject Focal Vertebral Brucellosis en_US
dc.subject Intervertebral Disk Degeneration en_US
dc.subject Kyphosis en_US
dc.subject Laminectomy en_US
dc.subject Mexican Americans en_US
dc.subject Milk en_US
dc.subject Open Reading Frame en_US
dc.subject Protein Microarray en_US
dc.subject Spinal Cord Compression en_US
dc.subject Spine en_US
dc.subject Spine Radiography en_US
dc.subject Spondylitis en_US
dc.subject Urine Retention en_US
dc.title Serial kinetics of the antibody response against the complete Brucella melitensis ORFeome in focal vertebral brucellosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.05298-11
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1098-660X


Ficheros en el ítem

Ficheros Tamaño Formato Ver

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

info:eu-repo/semantics/restrictedAccess Excepto si se señala otra cosa, la licencia del ítem se describe como info:eu-repo/semantics/restrictedAccess

Buscar en el Repositorio


Listar

Panel de Control

Estadísticas