A dot-ELISA using a partially purified cathepsin-L-like protein fraction from Taenia solium cysticerci, for the diagnosis of human neurocysticercosis

García Lescano, Héctor Hugo; Sifuentes, C.; Piña, R.; Gilman, Robert Hugh; Gutiérrez, A.H.; Rodriguez, S.; Sheen Cortavarria, Patricia; Zimic-Peralta, Mirko Juan; Rueda, D.; Flores, M.

dc.contributor.author	Piña, R.
dc.contributor.author	Gutiérrez, A.H.
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Rueda, D.
dc.contributor.author	Sifuentes, C.
dc.contributor.author	Flores, M.
dc.contributor.author	Sheen Cortavarria, Patricia
dc.contributor.author	Rodriguez, S.
dc.contributor.author	García Lescano, Héctor Hugo
dc.contributor.author	Zimic-Peralta, Mirko Juan
dc.date.accessioned	2022-01-18T19:34:35Z
dc.date.available	2022-01-18T19:34:35Z
dc.date.issued	2011
dc.identifier.uri	https://hdl.handle.net/20.500.12866/11028
dc.description.abstract	Human neurocysticercosis (NCC), caused by the cestode Taenia solium, is responsible for a significant amount of neurological morbidity and epilepsy in developing countries. The disease remains highly endemic in many areas, despite several efforts and interventions to control it. A simple, cheap and fast diagnostic assay that is suitable for use in field conditions is highly desired. In immunodiagnostics based on western immunoblots or standard ELISA, a cathepsin-L-like protein purified from the cysticercus fluid has previously performed well as an antigen. In a recent study in Peru, the same 53/25-kDa antigen was therefore used in the development of a dot-ELISA that could be employed for mass screenings under field conditions. The assay was standardized and tested not only against sera from a large group of NCC cases but also against sera from patients with other common parasitic infections, so that sensitivity and specificity could be assessed. For NCC, the assay gave better sensitivity in the detection of individuals with extraparenchymal cysts (94.4%-100%) or multiple parenchymal cysts (74.6%-80.0%) than in the detection of individuals with single parenchymal cysts (29.4%-45.1%). The assay also showed a high specificity for NCC (99.0%-100%), with a very low level of cross-reactivity with other parasitic infections. The dot-ELISA developed in this study is a highly specific, simple, cheap and rapid test for NCC that could be used under field conditions, even in the low-resource settings that are common in developing countries.	en_US
dc.language.iso	eng
dc.publisher	Taylor and Francis
dc.relation.ispartofseries	Annals of Tropical Medicine and Parasitology
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Humans	en_US
dc.subject	Animals	en_US
dc.subject	mass screening	en_US
dc.subject	Reproducibility of Results	en_US
dc.subject	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject	Western blotting	en_US
dc.subject	Sensitivity and Specificity	en_US
dc.subject	diagnostic test accuracy study	en_US
dc.subject	enzyme linked immunosorbent assay	en_US
dc.subject	Taenia solium	en_US
dc.subject	taeniasis	en_US
dc.subject	blood sampling	en_US
dc.subject	Cestoda	en_US
dc.subject	Antigens, Helminth	en_US
dc.subject	Cathepsin L	en_US
dc.subject	Cross Reactions	en_US
dc.subject	Cysticercus	en_US
dc.subject	Neurocysticercosis	en_US
dc.subject	Observer Variation	en_US
dc.subject	protein purification	en_US
dc.subject	Reagent Strips	en_US
dc.title	A dot-ELISA using a partially purified cathepsin-L-like protein fraction from Taenia solium cysticerci, for the diagnosis of human neurocysticercosis	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1179/136485911X12987676649782
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn	1364-8594