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Comparative gene expression analysis throughout the life cycle of Leishmania braziliensis: Diversity of expression profiles among clinical isolates

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dc.contributor.author Adaui, V.
dc.contributor.author Castillo Pareja, Denis Helan
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Gutierrez, Andrés
dc.contributor.author Decuypere, S.
dc.contributor.author Vanaerschot, M.
dc.contributor.author de Doncker, S.
dc.contributor.author Schnorbusch, K.
dc.contributor.author Maes, I.
dc.contributor.author van der Auwera, G.
dc.contributor.author Maes, L.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.contributor.author Dujardin, J.-C.
dc.date.accessioned 2022-01-18T19:34:37Z
dc.date.available 2022-01-18T19:34:37Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/11067
dc.description.abstract Background: Most of the Leishmania genome is reported to be constitutively expressed during the life cycle of the parasite, with a few regulated genes. Inter-species comparative transcriptomics evidenced a low number of species-specific differences related to differentially distributed genes or the differential regulation of conserved genes. It is of uppermost importance to ensure that the observed differences are indeed species-specific and not simply specific of the strains selected for representing the species. The relevance of this concern is illustrated by current study. Methodology/Principal Findings: We selected 5 clinical isolates of L. braziliensis characterized by their diversity of clinical and in vitro phenotypes. Real-time quantitative PCR was performed on promastigote and amastigote life stages to assess gene expression profiles at seven time points covering the whole life cycle. We tested 12 genes encoding proteins with roles in transport, thiol-based redox metabolism, cellular reduction, RNA poly(A)-tail metabolism, cytoskeleton function and ribosomal function. The general trend of expression profiles showed that regulation of gene expression essentially occurs around the stationary phase of promastigotes. However, the genes involved in this phenomenon appeared to vary significantly among the isolates considered. Conclusion/Significance: Our results clearly illustrate the unique character of each isolate in terms of gene expression dynamics. Results obtained on an individual strain are not necessarily representative of a given species. Therefore, extreme care should be taken when comparing the profiles of different species and extrapolating functional differences between them. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject animal cell en_US
dc.subject genetics en_US
dc.subject mouse en_US
dc.subject nucleotide sequence en_US
dc.subject Molecular Sequence Data en_US
dc.subject DNA, Protozoan en_US
dc.subject Leishmania braziliensis en_US
dc.subject gene expression regulation en_US
dc.subject Gene Expression Regulation en_US
dc.subject Sequence Analysis, DNA en_US
dc.subject amastigote en_US
dc.subject Reverse Transcriptase Polymerase Chain Reaction en_US
dc.subject real time polymerase chain reaction en_US
dc.subject Protozoan Proteins en_US
dc.subject growth, development and aging en_US
dc.subject protozoal DNA en_US
dc.subject protozoal protein en_US
dc.subject genetic variability en_US
dc.subject molecular genetics en_US
dc.subject reverse transcription polymerase chain reaction en_US
dc.subject chemistry en_US
dc.subject sequence alignment en_US
dc.subject DNA sequence en_US
dc.subject biosynthesis en_US
dc.subject parasite isolation en_US
dc.subject Gene Expression Profiling en_US
dc.subject promastigote en_US
dc.subject housekeeping gene en_US
dc.subject cytoskeleton en_US
dc.subject life cycle assessment en_US
dc.subject protein transport en_US
dc.subject RNA metabolism en_US
dc.title Comparative gene expression analysis throughout the life cycle of Leishmania braziliensis: Diversity of expression profiles among clinical isolates en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0001021
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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