Universidad Peruana Cayetano Heredia

Field Evaluation of a Coproantigen Detection Test for Fascioliasis Diagnosis and Surveillance in Human Hyperendemic Areas of Andean Countries

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dc.contributor.author Valero, M.A.
dc.contributor.author Periago, M.V.
dc.contributor.author Pérez-Crespo, I.
dc.contributor.author Angles, R.
dc.contributor.author Villegas, F.
dc.contributor.author Aguirre, C.
dc.contributor.author Strauss, W.
dc.contributor.author Espinoza, J.R.
dc.contributor.author Herrera Velit, Rosa Patricia
dc.contributor.author Terashima, A.
dc.contributor.author Tamayo, H.
dc.contributor.author Engels, D.
dc.contributor.author Gabrielli, A.F.
dc.contributor.author Mas-Coma, S.
dc.date.accessioned 2022-01-18T19:34:38Z
dc.date.available 2022-01-18T19:34:38Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/11080
dc.description.abstract Background: Emergence of human fascioliasis prompted a worldwide control initiative including a pilot study in a few countries. Two hyperendemic areas were chosen: Huacullani, Northern Altiplano, Bolivia, representing the Altiplanic transmission pattern with high prevalences and intensities; Cajamarca valley, Peru, representing the valley pattern with high prevalences but low intensities. Coprological sample collection, transport and study procedures were analyzed to improve individual diagnosis and subsequent treatments and surveillance activities. Therefore, a coproantigen-detection technique (MM3-COPRO ELISA) was evaluated, using classical techniques for egg detection for comparison. Methodology and Findings: A total of 436 and 362 stool samples from schoolchildren of Huacullani and Cajamarca, respectively, were used. Positive samples from Huacullani were 24.77% using the MM3-COPRO technique, and 21.56% using Kato-Katz. Positive samples from Cajamarca were 11.05% using MM3-COPRO, and 5.24% using rapid sedimentation and Kato-Katz. In Huacullani, using Kato-Katz as gold standard, sensitivity and specificity were 94.68% and 98.48%, respectively, and using Kato-Katz and COPRO-ELISA test together, they were 95.68% and 100%. In Cajamarca, using rapid sedimentation and Kato-Katz together, results were 94.73% and 93.58%, and using rapid sedimentation, Kato-Katz and copro-ELISA together, they were 97.56% and 100%, respectively. There was no correlation between coproantigen detection by optical density (OD) and infection intensity by eggs per gram of feces (epg) in Cajamarca low burden cases (<400 epg), nor in Huacullani high burden cases (≥400 epg), although there was in Huacullani low burden cases (<400 epg). Six cases of egg emission appeared negative by MM3-COPRO, including one with a high egg count (1248 epg). Conclusions: The coproantigen-detection test allows for high sensitivity and specificity, fast large mass screening capacity, detection in the chronic phase, early detection of treatment failure or reinfection in post-treated subjects, and usefulness in surveillance programs. However, this technique falls short when evaluating the fluke burden on its own. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject |endemic disease en_US
dc.subject disease severity en_US
dc.subject prevalence en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject Feces en_US
dc.subject Sensitivity and Specificity en_US
dc.subject infection rate en_US
dc.subject Fasciola hepatica en_US
dc.subject Fascioliasis en_US
dc.subject parasite identification en_US
dc.subject diagnostic test accuracy study en_US
dc.subject Bolivia en_US
dc.subject disease surveillance en_US
dc.subject enzyme linked immunosorbent assay en_US
dc.subject feces analysis en_US
dc.subject Antigens, Helminth en_US
dc.subject Mass Screening en_US
dc.subject Specimen Handling en_US
dc.subject antigen detection en_US
dc.subject Clinical Laboratory Techniques en_US
dc.subject Parasitology en_US
dc.subject optical density en_US
dc.subject coproantigen detection test en_US
dc.title Field Evaluation of a Coproantigen Detection Test for Fascioliasis Diagnosis and Surveillance in Human Hyperendemic Areas of Andean Countries en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0001812
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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