Universidad Peruana Cayetano Heredia

Non-invasive cytology brush pcr diagnostic testing in mucosal leishmaniasis: Superior performance to conventional biopsy with histopathology

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dc.contributor.author Boggild, A.K.
dc.contributor.author Valencia, B.M.
dc.contributor.author Veland, N.
dc.contributor.author Ramos, A.P.
dc.contributor.author Calderon, F.
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.contributor.author Low, D.E.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.date.accessioned 2022-01-18T19:34:39Z
dc.date.available 2022-01-18T19:34:39Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/11094
dc.description.abstract Background: Traditional methods of diagnosing mucosal leishmaniasis (ML), such as biopsy with histopathology, are insensitive and require collection of an invasive diagnostic specimen. Methods: We compared standard invasive procedures including biopsy histopathology, biopsy PCR, and leishmanin skin test (LST) to a novel, non-invasive, cytology-brush based PCR for the diagnosis of ML in Lima, Peru. Consensus reference standard was 2/4 tests positive, and outcome measures were sensitivity and specificity. Leishmania species identification was performed by PCR-based assays of positive specimens. Results: Twenty-eight patients were enrolled, 23 of whom fulfilled criteria for a diagnosis of ML. Sensitivity and specificity of biopsy with histopathology were 21.7% [95% CI 4.9-38.5%] and 100%; 69.6% [95% CI 50.8-88.4%] and 100% for LST; 95.7% [95% CI 87.4-100%] and 100% for biopsy PCR; and 95.7% [95% CI 87.4-100%] and 90% [95% CI 71.4-100%] for cytology brush PCR using both Cervisoft® and Histobrush® cervical cytology brushes. Represented species identified by PCR-RFLP included: L. (V). braziliensis (n = 4), and L. (V). peruviana (n = 3). Conclusions: Use of commercial grade cytology brush PCR for diagnosis of ML is sensitive, rapid, well tolerated, and carries none of the risks of invasive diagnostic procedures such as biopsy. Further optimization is required for adequate species identification. Further evaluation of this method in field and other settings is warranted. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject Biopsy en_US
dc.subject comparative study en_US
dc.subject controlled study en_US
dc.subject human tissue en_US
dc.subject nose mucosa en_US
dc.subject polymerase chain reaction en_US
dc.subject methodology en_US
dc.subject clinical article en_US
dc.subject Leishmania braziliensis en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject skin leishmaniasis en_US
dc.subject histopathology en_US
dc.subject molecular diagnosis en_US
dc.subject Sensitivity and Specificity en_US
dc.subject cytology en_US
dc.subject parasite identification en_US
dc.subject diagnostic test accuracy study en_US
dc.subject standard en_US
dc.subject controlled clinical trial en_US
dc.subject restriction fragment length polymorphism en_US
dc.subject Leishmania en_US
dc.subject Leishmania lainsoni en_US
dc.subject leishmaniasis en_US
dc.subject clinical trial en_US
dc.subject intermethod comparison en_US
dc.subject leishmania peruviana en_US
dc.subject Leishmaniasis, Mucocutaneous en_US
dc.subject Skin Tests en_US
dc.subject mouth mucosa en_US
dc.subject uterine cervix cytology en_US
dc.subject Molecular Diagnostic Techniques en_US
dc.subject non invasive measurement en_US
dc.subject instrumentation en_US
dc.subject cheek mucosa en_US
dc.subject mucosal leishmaniasis en_US
dc.subject pharynx en_US
dc.title Non-invasive cytology brush pcr diagnostic testing in mucosal leishmaniasis: Superior performance to conventional biopsy with histopathology en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0026395
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.00
dc.relation.issn 1932-6203


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