DSpace Repository

A diagnostic accuracy study of Xpert®MTB/RIF in HIV-positive patients with high clinical suspicion of pulmonary tuberculosis in Lima, Peru.

Show simple item record

dc.contributor.author Carriquiry, Gabriela
dc.contributor.author Otero Vegas, Larissa
dc.contributor.author González Lagos, Elsa Violeta
dc.contributor.author Zamudio Fuertes, Carlos Eduardo
dc.contributor.author Sánchez, Eduardo
dc.contributor.author Nabeta, Pamela
dc.contributor.author Campos, Miguel
dc.contributor.author Echevarria Zarate, Juan Ignacio
dc.contributor.author Seas Ramos, Carlos Rafael
dc.contributor.author Gotuzzo Herencia, José Eduardo
dc.date.accessioned 2022-01-18T19:34:39Z
dc.date.available 2022-01-18T19:34:39Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/11104
dc.description.abstract Background: Diagnosis of pulmonary tuberculosis (TB) among human immunodeficiency virus (HIV) patients remains complex and demands easy to perform and accurate tests. Xpert®MTB/RIF (MTB/RIF) is a molecular TB diagnostic test which is rapid and convenient; the test requires minimal human resources and reports results within two hours. The majority of performance studies of MTB/RIF have been performed in high HIV burden settings, thus TB diagnostic studies among HIV patients in low HIV prevalence settings such as Peru are still needed. METHODOLOGY/PRINCIPAL FINDINGS: From April to May , HIV-positive patients with high clinical suspicion of TB were enrolled from two tertiary hospitals in Lima, Peru. Detection of TB by MTB/RIF was compared to a composite reference standard Löwenstein-Jensen (LJ) and liquid culture. Detection of rifampicin resistance was compared to the LJ proportion method. We included 131 patients, the median CD4 cell count was 154.5 cells/mm(3) and 45 (34.4%) had TB. For TB detection among HIV patients, sensitivity of MTB/RIF was 97.8% (95% CI 88.4-99.6) (44/45); specificity was 97.7% (95% CI 91.9-99.4) (84/86); the positive predictive value was 95.7% (95% CI 85.5-98.8) (44/46); and the negative predictive value, 98.8% (95% CI 93.6-99.8) (84/85). MTB/RIF detected 13/14 smear-negative TB cases, outperforming smear microscopy [97.8% (44/45) vs. 68.9% (31/45); p = 0.0002]. For rifampicin resistance detection, sensitivity of MTB/RIF was 100% (95% CI 61.0-100.0) (6/6); specificity was 91.0% (95% CI 76.4-96.9) (30/33); the positive predictive value was 66.7% (95% CI 35.4-87.9) (6/9); and the negative predictive value was 100% (95% CI 88.7 -100.0) (30/30). CONCLUSIONS/SIGNIFICANCE: In HIV patients in our population with a high clinical suspicion of TB, MTB/RIF performed well for TB diagnosis and outperformed smear microscopy. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject article en_US
dc.subject controlled study en_US
dc.subject major clinical study en_US
dc.subject diagnostic test en_US
dc.subject sex difference en_US
dc.subject rifampicin en_US
dc.subject HIV Infections en_US
dc.subject Human immunodeficiency virus en_US
dc.subject molecular diagnosis en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Sputum en_US
dc.subject Human immunodeficiency virus infected patient en_US
dc.subject bacterium culture en_US
dc.subject diagnostic test accuracy study en_US
dc.subject lung tuberculosis en_US
dc.subject sputum smear en_US
dc.subject Tuberculosis, Pulmonary en_US
dc.subject CD4 lymphocyte count en_US
dc.subject outcome assessment en_US
dc.subject Antitubercular Agents en_US
dc.subject ethambutol en_US
dc.subject isoniazid en_US
dc.subject pyrazinamide en_US
dc.subject diagnostic accuracy en_US
dc.subject Rifampin en_US
dc.subject predictive value en_US
dc.subject microscopy en_US
dc.subject Lowenstein Jensen culture en_US
dc.subject Lowenstein Jensen proportion method en_US
dc.subject MTB RIF assay en_US
dc.subject mycobacteria growth indicator tube en_US
dc.title A diagnostic accuracy study of Xpert®MTB/RIF in HIV-positive patients with high clinical suspicion of pulmonary tuberculosis in Lima, Peru. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0044626
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.00
dc.relation.issn 1932-6203


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics